Complete Myogenic Differentiation of Adipogenic Stem Cells Requires Both Biochemical and Mechanical Stimulation
| Autor: | Thomas Aper, Mathias Wilhelmi, Florian Helms, Ulrike Böer, Melanie Klingenberg, Axel Haverich, Skadi Lau |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Phosphorylcholine Myocytes Smooth Muscle 0206 medical engineering Calponin Biomedical Engineering Pulsatile flow Stimulation Bone Morphogenetic Protein 4 02 engineering and technology Collagen Type I Contractility Andrology Bioreactors Sphingosine Transforming Growth Factor beta Physical Stimulation Pressure Humans Actin Aged Fibrin Adipogenesis Tissue Engineering Tissue Scaffolds biology Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Middle Aged 020601 biomedical engineering biology.protein Smoothelin Female Stress Mechanical Stem cell Tunica Media |
| Zdroj: | Annals of Biomedical Engineering. 48:913-926 |
| ISSN: | 1573-9686 0090-6964 |
| DOI: | 10.1007/s10439-019-02234-z |
| Popis: | Vascular tissue engineering of the middle layer of natural arteries requires contractile smooth muscle cells (SMC) which can be differentiated from adipose-derived mesenchymal stem cells (ASC) by treatment with transforming growth factor-β, sphingosylphosphorylcholine and bone morphogenetic protein-4 (TSB). Since mechanical stimulation may support or replace TSB-driven differentiation, we investigated its effect plus TSB-treatment on SMC orientation and contractile protein expression. Tubular fibrin scaffolds with incorporated ASC or pre-differentiated SMC were exposed to pulsatile perfusion for 10 days with or without TSB. Statically incubated scaffolds served as controls. Pulsatile incubation resulted in collagen-I expression and orientation of either cell type circumferentially around the lumen as shown by alpha smooth muscle actin (αSMA), calponin and smoothelin staining as early, intermediate and late marker proteins. Semi-quantitative Westernblot analyses revealed strongly increased αSMA and calponin expression by either pulsatile (12.48-fold; p |
| Databáze: | OpenAIRE |
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