Evolution of a culture protocol for successful blastocyst development and pregnancy
| Autor: | Annette Kausche, Gayle M Jones, Alan O Trounson, Nicholas Lolatgis, E Carl Wood, David K. Gardner |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Pregnancy Rate medicine.medical_treatment Pregnancy High-Risk Fertilization in Vitro Biology Culture Media Serum-Free Andrology Embryonic and Fetal Development Pregnancy Culture Techniques medicine Humans Blastocyst In vitro fertilisation Zygote Cell-Free System Rehabilitation Embryogenesis Obstetrics and Gynecology Embryo Embryo culture Embryo transfer Pregnancy rate medicine.anatomical_structure Treatment Outcome Reproductive Medicine embryonic structures Immunology Female Maternal Age |
| Zdroj: | Monash University |
| ISSN: | 0268-1161 |
| Popis: | A cell-free culture system was designed for human embryo development to the blastocyst stage by testing a range of culture conditions in a series of protocols. The culture system that was evolved has a brief 1 h exposure to spermatozoa and then culture of the pronucleate zygote for 2 days in IVF-50 medium. Two or three embryos were cultured together in 20 microl microdrops of medium under oil. Embryos were then regrouped and two or three at a similar stage were cultured together in 50 microl microdrops of Gardner's G2 medium under oil from days 3 to 5. Embryos were transferred to fresh G2 medium on day 5 and cultured for a further 1 or 2 days (day 6 or 7). No serum was used in any of the cultures. The embryo transfer medium and G2 medium were supplemented with human serum albumin. The zonae of all blastocysts to be transferred to patients were completely removed enzymatically. Using this protocol, 52% of zygotes developed to blastocysts and 34 out of 35 patients treated received 82 blastocysts and 11 morulae on day 5 or 6. Twenty-one fetal sacs with positive heartbeats (23% implantation rate) were detected in 13 ongoing pregnancies (38% pregnancy rate/transfer or 37%/patient treated). We anticipate that further improvements in embryo development and the selection of viable embryos can be achieved using this simple and effective culture system. |
| Databáze: | OpenAIRE |
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