Ecosystem screening approach for pathogen-associated microorganisms affecting host disease

Autor: Eric Galiana, Michel Ponchet, Gilles Arbiol, Benoit Industri, Catherine Mura, Antoine Marais
Přispěvatelé: Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
sporulation
PHYTOPHTHORA PARASITICA
PHOMA
VORTICELLA
RELATION HOTE-PARASITE
HOST PATHOGEN INTERACTIONS
MICROORGANISM
BIOLOGICAL ASSOCIATION
BIOLOGICAL CONTROL
GERMINATION INHIBITION
champignon phytopathogène
lutte biologique
Applied Microbiology and Biotechnology
Plant Roots
biofilm
Methods
Pathogen
Soil Microbiology
Oomycete
[SDV.EE]Life Sciences [q-bio]/Ecology
environment
Rhizosphere
Ecology
plante industrielle
inhibition
Phytophthora
rhizosphère
Biotechnology
Molecular Sequence Data
nicotiana tabacum
flore microbienne
Biology
Microbiology
pcr
Ascomycota
Tobacco
microbiologie
Ecosystem
Plant Diseases
Genetic diversity
Host (biology)
screening
fungi
Biofilm
Sequence Analysis
DNA
biology.organism_classification
tabac
Microbial population biology
Biofilms
Oligohymenophorea
Microbial Interactions
Food Science
Zdroj: Applied and Environmental Microbiology
Applied and Environmental Microbiology, American Society for Microbiology, 2011, 77 (17), pp.6069-6075. ⟨10.1128/AEM.05371-11⟩
Applied and Environmental Microbiology 17 (77), 6069-6075. (2011)
ISSN: 0099-2240
1098-5336
DOI: 10.1128/AEM.05371-11⟩
Popis: The microbial community in which a pathogen evolves is fundamental to disease outcome. Species interacting with a pathogen on the host surface shape the distribution, density, and genetic diversity of the inoculum, but the role of these species is rarely determined. The screening method developed here can be used to characterize pathogen-associated species affecting disease. This strategy involves three steps: (i) constitution of the microbial community, using the pathogen as a trap; (ii) community selection, using extracts from the pathogen as the sole nutrient source; and (iii) molecular identification and the screening of isolates focusing on their effects on the growth of the pathogen in vitro and host disease. This approach was applied to a soilborne plant pathogen, Phytophthora parasitica , structured in a biofilm, for screening the microbial community from the rhizosphere of Nicotiana tabacum (the host). Two of the characterized eukaryotes interfered with the oomycete cycle and may affect the host disease. A Vorticella species acted through a mutualistic interaction with P. parasitica , disseminating pathogenic material by leaving the biofilm. A Phoma species established an amensal interaction with P. parasitica , strongly suppressing disease by inhibiting P. parasitica germination. This screening method is appropriate for all nonobligate pathogens. It allows the definition of microbial species as promoters or suppressors of a disease for a given biotope. It should also help to identify important microbial relationships for ecology and evolution of pathogens.
Databáze: OpenAIRE
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