Molecular characterization and immune protection by cystathionine β‐synthase fromEimeria tenella
| Autor: | Hui Dong, Hongyu Han, Huanzhi Zhao, Shunhai Zhu, Bing Huang, Cong Li, Qiping Zhao |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Protozoan Proteins
Cystathionine beta-Synthase Transsulfuration pathway Microbiology Eimeria law.invention Western blot law Complementary DNA parasitic diseases medicine Animals Poultry Diseases biology medicine.diagnostic_test Coccidiosis Oocysts medicine.disease biology.organism_classification Molecular biology Cystathionine beta synthase Recombinant Proteins Sporozoites Polyclonal antibodies Recombinant DNA biology.protein Rabbits Chickens Eimeria tenella |
| Zdroj: | Journal of Eukaryotic Microbiology. 69 |
| ISSN: | 1550-7408 1066-5234 |
| DOI: | 10.1111/jeu.12876 |
| Popis: | Eimeria tenella is an obligate intracellular apicomplexan parasite that causes avian coccidiosis and leads to severe economic losses in the global poultry industry. Cystathionine β-synthase (CBS) and cystathionine γ-lyase (CGL) act together to generate H2S in the reverse transsulfuration pathway. In this study, E. tenella Cystathionine β-synthase (EtCBS) was cloned using rapid amplification of cDNA 5'-ends (5'RACE) and characterized, and its immunoprotective effects were evaluated. The recombinant EtCBS protein (rEtCBS) was expressed and successfully recognized by anti-sporozoites (Spz) protein rabbit serum. EtCBS mRNA levels were highest in Spz by qPCR, and the protein expression levels were higher in unsporulated oocysts (UO) than in other stages by Western blot. Indirect immunofluorescence showed that EtCBS protein was found on the surface of Spz and second-generation merozoites (Mrz). The invasion inhibition assays showed that rabbit anti-rEtCBS polyclonal antibodies effectively inhibited parasite invasion host cells. Chickens immunized with rEtCBS protein showed prominently increased weight gains and decreased oocyst output compared to nonimmunized and infected control group. The results suggest that EtCBS could be a potential vaccine candidate against E. tenella. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text