Effect of the peptide bronchogen (Ala-Asp-Glu-Leu) on DNA thermostability
| Autor: | D. G. Khachidze, E. M. Lomidze, T Lezhava, J. Monaselidze, Tinatin Jokhadze, M. Z. Gorgoshidze, V. Kh. Khavinson |
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| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Calorimetry Differential Scanning Chemistry Melting temperature Enthalpy Peptide General Medicine Ligand (biochemistry) Nucleic Acid Denaturation General Biochemistry Genetics and Molecular Biology Nucleic acid thermodynamics chemistry.chemical_compound Crystallography Mice Biochemistry Ala-Asp-Glu-Leu Animals Transition Temperature Cattle Peptides DNA Thermostability |
| Zdroj: | Bulletin of experimental biology and medicine. 150(3) |
| ISSN: | 1573-8221 |
| Popis: | Thermodynamic parameters of DNA melting in the presence of a peptide bronchogen in various concentrations were estimated on a differential scanning microcalorimeter. Bronchogen was shown to serve as a DNA-stabilizing agent. Bronchogen increased the melting temperature of DNA from calf thymus and mouse liver by 3.1°C in a narrow range of r (molar ratio of bronchogen/DNA b.p., 0.01-0.055). A further increase in r was not accompanied by changes in the melting temperature. The complex melting enthalpy (ΔH melt ) remained unchanged in this range of r (0.01-1.0). ΔH melt for DNA from the thymus and mouse liver was 11.4 and 12.7 cal/g, respectively. Our results indicate that bronchogen is not an adenine–thymine-specific or guanine–cytosine-specific ligand. The type of binding is considered as strong and occasional. The binding occurs with both strands of DNA (mainly with nitrogen bases). |
| Databáze: | OpenAIRE |
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