Imidazole metalloporphyrins as photosensitizers for photodynamic therapy: Role of molecular charge, central metal and hydroxyl radical production
| Autor: | Zahra Kamal, Pawel Mroz, Michael R. Hamblin, David F. Bocian, Laiqua Khalid, Hooi Ling Kee, Jayeeta Bhaumik, Dilek Kiper Dogutan, Zarmeneh Aly, Jonathan S. Lindsey, Dewey Holten |
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| Rok vydání: | 2009 |
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Molecular Cancer Research Metalloporphyrins Radical medicine.medical_treatment Apoptosis Photodynamic therapy Adenocarcinoma Photochemistry Article HeLa Mice chemistry.chemical_compound Cell Line Tumor polycyclic compounds medicine Animals Humans Photosensitizer chemistry.chemical_classification Reactive oxygen species biology Hydroxyl Radical Imidazoles Cationic polymerization biology.organism_classification Porphyrin Mitochondria Microscopy Fluorescence Photochemotherapy Oncology Biochemistry chemistry Colonic Neoplasms Hydroxyl radical Lysosomes HeLa Cells |
| Zdroj: | Cancer Letters. 282:63-76 |
| ISSN: | 0304-3835 |
| Popis: | The in vitro photodynamic therapy activity of four imidazole-substituted metalloporphyrins has been studied using human (HeLa) and mouse (CT26) cancer cell lines: an anionic Zn porphyrin and a homologous series of three cationic Zn, Pd or InCl porphyrins. A dramatic difference in phototoxicity was found: Pd cationic > InCl cationic > Zn cationic > Zn anionic. HeLa cells were more susceptible than CT26 cells. Induction of apoptosis was demonstrated using a fluorescent caspase assay. The anionic Zn porphyrin localized in lysosomes while the cationic Zn porphyrin localized in lysosomes and mitochondria, as assessed by fluorescence microscopy. Studies using fluorescent probes suggested that the cationic Pd porphyrin produced more hydroxyl radicals as the reactive oxygen species. Thus, the cationic Pd porphyrin has high potential as a photosensitizer and gives insights into characteristics for improved molecular designs. |
| Databáze: | OpenAIRE |
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