Linkage mapping in the oilseed crop Jatropha curcas L. reveals a locus controlling the biosynthesis of phorbol esters which cause seed toxicity
| Autor: | Y. K. Tripathi, Andrew J. King, Luis R. Montes, Jasper G. Clarke, Ian A. Graham, Piet van der Linde, Evanilda Tavares, Eibertus N. van Loo, Hanneke Witsenboer, Julie Affleck, Yi Li, Parul Shukla, Thirunavukkarasu Rajasekaran, Edwin A. G. van der Vossen |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Genetic Markers construction linkage mapping Genetic Linkage biofuel plant Jatropha markers Locus (genetics) Plant Science Biology Quantitative trait locus oil 01 natural sciences Polymorphism Single Nucleotide Chromosomes Plant 03 medical and health sciences transcriptome analysis PPO Mushrooms Laboratorium voor Plantenveredeling Gene mapping Genetic linkage Jatropha curcas Botany Plant breeding genome Research Articles 030304 developmental biology 2. Zero hunger Genetics oilseed 0303 health sciences phorbol esters PPO Paddestoelen maps qtl Chromosome Mapping genetic diversity sequence biology.organism_classification Plant Breeding Genetic marker Seeds Agronomy and Crop Science 010606 plant biology & botany Biotechnology Microsatellite Repeats |
| Zdroj: | Plant Biotechnology Journal Plant Biotechnology Journal 11 (2013) 8 Plant Biotechnology Journal, 11(8), 986-996 |
| ISSN: | 1467-7644 |
| Popis: | Current efforts to grow the tropical oilseed crop Jatropha curcas L. economically are hampered by the lack of cultivars and the presence of toxic phorbol esters (PE) within the seeds of most provenances. These PE restrict the conversion of seed cake into animal feed, although naturally occurring ‘nontoxic’ provenances exist which produce seed lacking PE. As an important step towards the development of genetically improved varieties of J. curcas, we constructed a linkage map from four F2 mapping populations. The consensus linkage map contains 502 codominant markers, distributed over 11 linkage groups, with a mean marker density of 1.8 cM per unique locus. Analysis of the inheritance of PE biosynthesis indicated that this is a maternally controlled dominant monogenic trait. This maternal control is due to biosynthesis of the PE occurring only within maternal tissues. The trait segregated 3 : 1 within seeds collected from F2 plants, and QTL analysis revealed that a locus on linkage group 8 was responsible for phorbol ester biosynthesis. By taking advantage of the draft genome assemblies of J. curcas and Ricinus communis (castor), a comparative mapping approach was used to develop additional markers to fine map this mutation within 2.3 cM. The linkage map provides a framework for the dissection of agronomic traits in J. curcas, and the development of improved varieties by marker-assisted breeding. The identification of the locus responsible for PE biosynthesis means that it is now possible to rapidly breed new nontoxic varieties. |
| Databáze: | OpenAIRE |
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