Molecular and clinical characterization of plasmid-mediated AmpC β-lactamase-producing Escherichia coli bacteraemia: a comparison with extended-spectrum β-lactamase-producing and non-resistant E. coli bacteraemia
| Autor: | Aki Matsushima, Toshiaki Komori, S. Takakura, Naohisa Fujita, Tetsuya Yagi, Miki Nagao, Yasufumi Matsumura, Masaki Yamamoto, Satoshi Ichiyama, Mitsutaka Iguchi |
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| Rok vydání: | 2013 |
| Předmět: |
AmpC β-lactamase
Serotype clone (Java method) Adult DNA Bacterial Male Microbiology (medical) sequence type Genotype medicine.drug_class Antibiotics bloodstream infection Bacteremia Biology medicine.disease_cause molecular epidemiology Polymerase Chain Reaction beta-Lactamases law.invention Microbiology Hospitals University Plasmid Japan law Risk Factors polycyclic compounds medicine Escherichia coli Humans Polymerase chain reaction Escherichia coli Infections Phylogeny Aged Molecular epidemiology General Medicine Sequence Analysis DNA biochemical phenomena metabolism and nutrition Middle Aged extended-spectrum β-lactamase bacterial infections and mycoses Virology Molecular Typing Infectious Diseases Case-Control Studies bacteria Female Plasmids |
| Zdroj: | Clinical Microbiology and Infection. 19(2):161-168 |
| ISSN: | 1198-743X |
| DOI: | 10.1111/j.1469-0691.2012.03762.x |
| Popis: | Plasmid-mediated AmpC β-lactamase-producing Escherichia coli (AmpC-E) bacteraemia was characterized by comparison with bacteraemia caused by extended-spectrum β-lactamase (ESBL)-producing E. coli (ESBL-E) and non-resistant E. coli (NR-E) in the era of the worldwide spread of the CTX-M-15-producing O25b-ST131-B2 clone. Of 706 bloodstream E. coli isolates collected between 2005 and 2010 in three Japanese university hospitals, 111 ESBL screening-positive isolates were analysed for AmpC and ESBL genes by PCR. A case–control study was performed in which the cases consisted of all of the patients with AmpC-E bacteraemia. Phylogenetic groups, sequence types and O25b serotype were determined. Twenty-seven AmpC-E isolates (26 of which were of the CMY-2 type) were identified, and 54 ESBL-E and 54 NR-E isolates were selected for the controls. Nineteen AmpC-E isolates were also positive for ESBL. CTX-M-14 was the most prevalent ESBL type among both the AmpC-E and ESBL-E isolates. The O25b-ST131-B2 clone was the most prevalent among the ESBL-E isolates (26%) and the second most prevalent among the NR-E isolates (13%), but only one O25b-ST131-B2 clone was found among the AmpC-E isolates. Twenty-three different sequence types were identified among the AmpC-E isolates. When compared with bacteraemia with ESBL-E, previous isolation of multidrug-resistant bacteria and intravascular catheterization were independently associated with a lower risk for AmpC-E. When compared with NR-E bacteraemia, prior use of antibiotics was the only significant risk factor for AmpC-E. Unlike the spread of the O25b-ST131-B2 clone between ESBL-E and NR-E, the AmpC-E isolates were not dominated by any specific clone. |
| Databáze: | OpenAIRE |
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