Effects of soybean antigen proteins on intestinal permeability, 5-hydroxytryptamine levels and secretory IgA distribution in the intestine of weaned piglets
Autor: | Yu Zhang, Shi Bin Feng, Xi Chun Wang, Jin Jie Wu, Liang You Ma, Ya Nan Kou, Yu Li, Dan Dan Ren, Cheng Ming Cao |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
animal diseases Biology piglets 5-hydroxytryptamine Andrology 03 medical and health sciences Basal (phylogenetics) fluids and secretions Antigen medicine Distribution (pharmacology) Secretory IgA lcsh:SF1-1100 030109 nutrition & dietetics Intestinal permeability integumentary system intestinal permeability 0402 animal and dairy science 04 agricultural and veterinary sciences medicine.disease 040201 dairy & animal science Small intestine Staining medicine.anatomical_structure secretory IgA Immunology Animal Science and Zoology soybean antigen protein lcsh:Animal culture Diamine oxidase |
Zdroj: | Italian Journal of Animal Science, Vol 15, Iss 1, Pp 174-180 (2016) |
Popis: | In this experiment, the effects of soybean antigen proteins on intestinal permeability, 5-hydroxytryptamine (5-HT) levels and secretory IgA (sIgA) distribution in the intestine of weaned piglets were evaluated. Thirty piglets (21 d of age) were randomly divided into three groups: control group, glycinin-sensitised group and β-conglycinin-sensitised group. Piglets in the control group were fed a basal diet, and in two sensitised groups were fed the diet with 4% glycinin or β-conglycinin. Piglets in glycinin and β-conglycinin groups were sensitised twice on 21–27 d and 32–34 d period, respectively. Blood samples were collected and analysed for D-lactic acid, diamine oxidase (DAO) and 5-HT levels on d 21, 28 and 35. At the end of the trial, five piglets per group were sacrificed and the small intestine was collected to evaluate intestinal sIgA distribution and the optical density (OD) of the relative staining positivity. The results revealed that the piglets in glycinin and β-conglycinin groups had higher D-lactic acid, DAO and 5-HT levels than those of the control group (p duodenum > proximal jejunum and distal ileum > distal jejunum. These results suggest that soybean antigen proteins induce allergic reactions, damage the intestinal mucosa, increase intestinal permeability and promote sIgA synthesis in weaned piglets. |
Databáze: | OpenAIRE |
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