SDF-9, a protein tyrosine phosphatase-like molecule, regulates the L3/dauer developmental decision through hormonal signaling inC. elegans
| Autor: | Kiyotaka Ohkura, Norio Suzuki, Takeshi Ishihara, Isao Katsura |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Complementary Time Factors Recombinant Fusion Proteins Green Fluorescent Proteins Molecular Sequence Data Mutant Protein tyrosine phosphatase Biology Dauer larva Models Biological Alae Genes Reporter Animals Tissue Distribution Amino Acid Sequence Transgenes Cloning Molecular Tyrosine Caenorhabditis elegans Caenorhabditis elegans Proteins Promoter Regions Genetic Molecular Biology Alleles Phylogeny Larva Base Sequence Models Genetic Sequence Homology Amino Acid fungi Temperature Embryonic stem cell Phenotype Hormones Cell biology Luminescent Proteins Cholesterol Microscopy Fluorescence Mutation Steroids Protein Tyrosine Phosphatases Signal Transduction Developmental Biology |
| Zdroj: | Development. 130:3237-3248 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | The dauer larva of the nematode Caenorhabditis elegans is a good model system for investigating the regulation of developmental fates by environmental cues. Here we show that SDF-9, a protein tyrosine phosphatase-like molecule, is involved in the regulation of dauer larva formation. The dauer larva of sdf-9 mutants is different from a normal dauer larva but resembles the dauer-like larva of daf-9 and daf-12 dauer-constitutive mutants. Like these mutants, the dauer-constitutive phenotypes of sdf-9 mutants were greatly enhanced by cholesterol deprivation. Epistasis analyses, together with the relationship between sdf-9 mutations and daf-9 expression, suggested that SDF-9 increases the activity of DAF-9 or helps the execution of the DAF-9 function. SDF-9 was expressed in two head cells in which DAF-9 is expressed. By their position and by genetic mosaic experiments, we identified these cells as XXXL/R cells, which are known as embryonic hypodermal cells and whose function at later stages is unknown. Killing of the sdf-9-expressing cells in the wild-type first-stage larva induced formation of the dauer-like larva. Since this study on SDF-9 and former studies on DAF-9 showed that the functions of these proteins are related to those of steroids, XXXL/R cells seem to play a key role in the metabolism or function of a steroid hormone(s)that acts in dauer regulation. |
| Databáze: | OpenAIRE |
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