Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments
| Autor: | Natalie Gindi, Efrat Har-Paz, Ruth Shalgi, Mattan Levi, Dalia Galiani, Hadas Grossman, Nava Dekel, Irit Miller, Nava Nevo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Science Cellular differentiation Biology Proto-Oncogene Proteins c-fyn Article Mice 03 medical and health sciences chemistry.chemical_compound FYN medicine Animals Humans Cytochalasin Microinjection Actin Cell Nucleus Analysis of Variance Multidisciplinary urogenital system Cell Differentiation Oocyte Actins Cell biology Meiosis MicroRNAs Cell nucleus 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation chemistry embryonic structures Oocytes Medicine Female RNA Interference Protein Multimerization Protein Processing Post-Translational Lamin |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-02071-x |
| Popis: | Meiotically arrested oocytes are characterized by the presence of the nuclear structure known as germinal-vesicle (GV), the breakdown of which (GVBD) is associated with resumption of meiosis. Fyn is a pivotal factor in resumption of the first meiotic division; its inhibition markedly decreases the fraction of oocytes undergoing GVBD. Here, we reveal that in mouse oocytes Fyn is post-transcriptionally regulated by miR-125a-3p. We demonstrate that in oocytes resuming meiosis miR-125a-3p and Fyn exhibit a reciprocal expression pattern; miR-125a-3p decreases alongside with an increase in Fyn expression. Microinjection of miR-125a-3p inhibits GVBD, an effect that is markedly reduced by Fyn over-expression, and impairs the organization of the actin rim surrounding the nucleus. Lower rate of GVBD is also observed in oocytes exposed to cytochalasin-D or blebbistatin, which interfere with actin polymerization and contractility of actin bundles, respectively. By down-regulating Fyn in HEK-293T cells, miR-125a-3p reduces the interaction between actin and A-type lamins, which constitute the nuclear-lamina. Our findings suggest a mechanism, by which a decrease in miR-125a-3p during oocyte maturation facilitates GVBD by allowing Fyn up-regulation and the resulting stabilization of the interaction between actin and A-type lamins. |
| Databáze: | OpenAIRE |
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