LMP1gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip
| Autor: | Nai-Cheng Hou, Chih-Shen Chuang, Chieh-Ying Wu, Pei-Kuen Wei, Po-Han Juan, Yu Jui Fan, Horn-Jiunn Sheen |
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| Rok vydání: | 2020 |
| Předmět: |
Herpesvirus 4
Human Nanostructure Materials science education Microfluidics Immobilized Nucleic Acids Nanowire Biosensing Techniques 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry Viral Matrix Proteins Limit of Detection Lab-On-A-Chip Devices Electrochemistry Environmental Chemistry Surface plasmon resonance Spectroscopy Detection limit Base Sequence Nanowires business.industry 010401 analytical chemistry Nucleic Acid Hybridization Microfluidic Analytical Techniques Surface Plasmon Resonance 021001 nanoscience & nanotechnology Chip 0104 chemical sciences DNA Viral Optoelectronics Gold DNA Probes 0210 nano-technology business Refractive index Biosensor |
| Zdroj: | The Analyst. 145:52-60 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c9an01419e |
| Popis: | Surface plasmon resonance (SPR) nanowire array chips with a microfluidic system are an effective detection method for a rapid test device. This study investigated a capped gold nanowire array and a microfluidic test platform to provide a fundamental understanding of the kinetic binding of SPR nanowires and the surface gold refractive index. The device sensitivity of the SPR nanowire array was 485 nm RIU-1 and the detection limit was 4.1 × 10-5 RIU. Moreover, a kinetic binding analysis also indicated that a peak shift resulted from a specific hybridization of the target molecule with the immobilized probe on the gold nanostructures. The peak shift (red-shift) value of latent membrane protein 1 (LMP1) DNA was 2.21 nm. The results demonstrated that this new method had high sensitivity to detect amplified DNA products without labeling or complex sample treatment. The SPR nanowire chip can detect the PCR products at lower cycle numbers compared to gel electrophoresis due to probe and DNA specificity. Furthermore, the mechanisms of SPR nanowire array fabrication and the detection of the LMP1 gene were studied. The findings can assist in improving the biosensing of DNA-amplified products and in developing rapid detection devices with a small-footprint nanostructured SPR chip. |
| Databáze: | OpenAIRE |
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