CLAMP/Spef1 regulates planar cell polarity signaling and asymmetric microtubule accumulation in the Xenopus ciliated epithelia
| Autor: | Mohamed M. Altabbaa, Eva J. Brotslaw, Michael E. Werner, Sun K. Kim, Jennifer W. Mitchell, Siwei Zhang, Brian J. Mitchell |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell division Xenopus Xenopus Proteins Microtubules Xenopus laevis 03 medical and health sciences Microtubule Report Cell polarity Ciliary rootlet Animals Cilia Cytoskeleton Research Articles biology Cilium Cell Membrane Wnt signaling pathway Cell Polarity Epithelial Cells Cell Biology biology.organism_classification respiratory tract diseases Cell biology Protein Transport 030104 developmental biology Microtubule-Associated Proteins Cell Division Signal Transduction |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201706058 |
| Popis: | Kim et al. show that CLAMP regulates planar cell polarity (PCP) signaling. Its depletion causes a loss of the atypical cadherin Celsr2, a loss of PCP protein asymmetry, and a defect in cilia polarity and oriented cell division. CLAMP also, via its role in PCP, regulates the accumulation of an asymmetric pool of microtubules. Most epithelial cells polarize along the axis of the tissue, a feature known as planar cell polarity (PCP). The initiation of PCP requires cell–cell signaling via the noncanonical Wnt/PCP pathway. Additionally, changes in the cytoskeleton both facilitate and reflect this polarity. We have identified CLAMP/Spef1 as a novel regulator of PCP signaling. In addition to decorating microtubules (MTs) and the ciliary rootlet, a pool of CLAMP localizes at the apical cell cortex. Depletion of CLAMP leads to the loss of PCP protein asymmetry, defects in cilia polarity, and defects in the angle of cell division. Additionally, depletion of CLAMP leads to a loss of the atypical cadherin-like molecule Celrs2, suggesting that CLAMP facilitates the stabilization of junctional interactions responsible for proper PCP protein localization. Depletion of CLAMP also affects the polarized organization of MTs. We hypothesize that CLAMP facilitates the establishment of cell polarity and promotes the asymmetric accumulation of MTs downstream of the establishment of proper PCP. |
| Databáze: | OpenAIRE |
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