Cancer-testis antigen expression is shared between epithelial ovarian cancer tumors
| Autor: | Wilberto Nieves-Neira, Natasa Strbo, Fiona Simpkins, Feng Miao, Eckhard R. Podack, Arlene E. Garcia-Soto, Tulay Koru-Sengul, Joseph A. Lucci, Parvin Ganjei-Azar, Taylor H. Schreiber |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Pathology medicine.medical_specialty endocrine system diseases Adolescent medicine.medical_treatment Carcinoma Ovarian Epithelial Cancer Vaccines Young Adult Cancer immunotherapy Antigen Antigens Neoplasm Cell Line Tumor medicine Humans Epithelial ovarian cancer Neoplasms Glandular and Epithelial RNA Neoplasm Aged Aged 80 and over Ovarian Neoplasms business.industry Obstetrics and Gynecology Immunotherapy Middle Aged medicine.disease female genital diseases and pregnancy complications Serous fluid Oncology Cell culture Cancer/testis antigens Female Neoplasm Grading business Ovarian cancer |
| Zdroj: | Gynecologic oncology. 145(3) |
| ISSN: | 1095-6859 |
| Popis: | Objectives Cancer-testis (CT) antigens have been proposed as potential targets for cancer immunotherapy. Our objective was to evaluate the expression of a panel of CT antigens in epithelial ovarian cancer (EOC) tumor specimens, and to determine if antigen sharing occurs between tumors. Methods RNA was isolated from EOC tumor specimens, EOC cell lines and benign ovarian tissue specimens. Real time-PCR analysis was performed to determine the expression level of 20 CT antigens. Results A total of 62 EOC specimens, 8 ovarian cancer cell lines and 3 benign ovarian tissues were evaluated for CT antigen expression. The majority of the specimens were: high grade (62%), serous (68%) and advanced stage (74%). 58 (95%) of the EOC tumors analyzed expressed at least one of the CT antigens evaluated. The mean number of CT antigen expressed was 4.5 (0-17). The most frequently expressed CT antigen was MAGE A4 (65%). Antigen sharing analysis showed the following: 9 tumors shared only one antigen with 62% of the evaluated specimens, while 37 tumors shared 4 or more antigens with 82%. 5 tumors expressed over 10 CT antigens, which were shared with 90% of the tumor panel. Conclusion CT antigens are expressed in 95% of EOC tumor specimens. However, not a single antigen was universally expressed across all samples. The degree of antigen sharing between tumors increased with the total number of antigens expressed. These data suggest a multi-epitope approach for development of immunotherapy for ovarian cancer treatment. |
| Databáze: | OpenAIRE |
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