Insights into the population structure of Mycobacterium tuberculosis using spoligotyping and RDRio in a southeastern Brazilian prison unit
| Autor: | Sidra Ezidio Gonçalves Vasconcellos, Angela Maria Werneck Barreto, Maria Helena Féres Saad, Véronique Massari, Fé Dagmar Huber, Vanderci Cesconi, Michel K. Gomgnimbou, Bernard Larouzé, Alexandra Sánchez, Philip Noel Suffys, Christophe Sola, Silvia Maria Almeida Machado, Harrison Magdinier Gomes |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Adult Veterinary medicine Tuberculosis Genotype media_common.quotation_subject Prison Biology Disease cluster Microbiology Polymorphism Single Nucleotide Mycobacterium tuberculosis Young Adult Gene Frequency Polymorphism (computer science) Genetics medicine Humans Typing Clade Molecular Biology Ecology Evolution Behavior and Systematics Alleles media_common Polymorphism Genetic Prisoners Middle Aged biology.organism_classification medicine.disease Infectious Diseases Prisons Brazil Polymorphism Restriction Fragment Length |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 26 |
| ISSN: | 1567-7257 |
| Popis: | Tuberculosis (TB) is still a serious public health problem, continuing to be an important threat for confined populations. We used spoligotyping to estimate the genotypic clades of Mycobacterium tuberculosis isolates from inmates in two blocks in a southeastern Brazilian prison unit, with TB incidence rate of 8185/100.000. The Latin American Mediterranean (LAM) clade is well represented in the country, and the LAM specific molecular markers, RD Rio large sequence polymorphism and the SNP on the Rv3062 [ligB 1212 ], were used to characterize spoligotype signatures from prison isolates. Typing of RD Rio and ligB increase LAM clade from 66.7% ( n = 72/108) to 69.4% ( n = 75). The LAM2 SIT17 ( n = 23) and SIT179 ( n = 12) signatures comprised one third of all isolates, followed by Haarlem (11.5%, n = 12), T (8.7%, n = 9) and X (5.7%, n = 6) clades. Strains with unknown signatures represented 5.5% ( n = 6), and four (3.7%) did not match any lineage. We observed RD Rio among 64 (59.2%) isolates, and 54 (50%) were of the LAM clade. In particular, the LAM2/RD Rio sub-lineage was significantly associated with clustering ( p = 0.02) and its frequency was higher (32%) when compared to that of the previous general TB cases in RJ (4.29%). Overall cluster frequency defined by spoligotyping/IS6110-RFLP was 62%. The two evolutionary markers helped to evaluate some LAM signature misconceptions and demonstrate that LAM2/RD Rio was found with high frequency, hitherto being unnoticed. All these data, allied to high clustering, imply that public health measures to minimize the escalation of TB in prison is essential, and both spoligotyping as well as RD Rio would be useful tools to monitor the effects of the measures with respect to M. tuberculosis lineage variation. |
| Databáze: | OpenAIRE |
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