SIV Infection of Monkey Spleen Cells Including Follicular Dendritic Cells in Different Stages of Disease
| Autor: | Cosme Ordonez, D. Böttiger, Ingrid Stahmer, Peter Biberfeld, Gunnel Biberfeld, Jan Albert, Marianne Ekman, Roberto Mesquita, Per Putkonen, Mikulas Popovic |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA Replication
Molecular Sequence Data Immunology Simian Acquired Immunodeficiency Syndrome Spleen Biology Virus Replication medicine.disease_cause Genes env Polymerase Chain Reaction Virus Virology medicine Splenocyte Animals Immunology and Allergy Cytotoxic T cell Lymphocytes RNA Messenger In Situ Hybridization DNA Primers Cytopathic effect Base Sequence Follicular dendritic cells Macrophages Dendritic Cells Dendritic cell Simian immunodeficiency virus Genes pol CD4 Lymphocyte Count Macaca fascicularis medicine.anatomical_structure DNA Viral Simian Immunodeficiency Virus |
| Zdroj: | Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 11:1-9 |
| ISSN: | 1077-9450 |
| Popis: | Immunoaffinity enriched spleen follicular dendritic cells (FDCs), lymphocytes, and macrophages from SIVsm-inoculated cynomolgus monkeys (Macaca fascicularis) at different stages of disease were compared for latent and productive SIV infection. Analysis of FDCs by in situ hybridization, electron microscopy, and coculture assays indicated that comparatively high levels of virus were associated with the FDC fraction. Polymerase chain reaction (PCR) and RT-PCR results revealed that the levels for SIVpol DNA did not correlate with the level of env mRNA in the various cell subsets, suggesting differences in latency. Limiting dilution assays for spliced env mRNA showed a 10-100-fold higher amount of env mRNA in FDCs than in other spleen cell subsets early during SIV infection. At late stages of disease, the number of productively infected FDCs significantly decreased in parallel with a marked reduction of the FDC network and follicular involution. Our findings indicate that destruction of FDCs probably reflects a cytopathic effect of SIV and/or the activity of specific antiviral cytotoxic T lymphocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|