Programmable molecular transport achieved by engineering protein motors to move on DNA nanotubes

Autor:	Ryota Ibusuki, Tatsuya Morishita, Akane Furuta, Shintaro Nakayama, Maki Yoshio, Hiroaki Kojima, Kazuhiro Oiwa, Ken’ya Furuta
Rok vydání:	2022
Předmět:	DNA-Binding Proteins Multidisciplinary Nanotubes Protein Domains Dyneins Nucleic Acid Conformation Biological Transport DNA Protein Engineering Article Protein Binding
Zdroj:	Science
ISSN:	1095-9203
Popis:	Intracellular transport is the basis of microscale logistics within cells and is powered by biomolecular motors. Mimicking transport for in vitro applications has been widely studied; however, the inflexibility in track design and control has hindered practical applications. Here, we developed protein-based motors that move on DNA nanotubes by combining a biomolecular motor dynein and DNA binding proteins. The new motors and DNA-based nanoarchitectures enabled us to arrange the binding sites on the track, locally control the direction of movement, and achieve multiplexed cargo transport by different motors. The integration of these technologies realized microscale cargo sorters and integrators that automatically transport molecules as programmed in DNA sequences on a branched DNA nanotube. Our system should provide a versatile, controllable platform for future applications.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04674870749ae4dcf68d5d0d95e82554 https://pubmed.ncbi.nlm.nih.gov/35271310 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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