The 5?-untranslated region sequence of a potential new genotype of bovine viral diarrhea virus
| Autor: | Youssef Elazhary, E. Cornaglia, R. H. Yolken, S. Mehdy Elahi, Serge Harpin |
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| Rok vydání: | 1995 |
| Předmět: |
Untranslated region
Genotype Five prime untranslated region animal diseases viruses Molecular Sequence Data Restriction Mapping Polymerase Chain Reaction complex mixtures Disease Outbreaks PstI Restriction map Sequence Homology Nucleic Acid Virology Animals Lung Base Sequence biology Diarrhea Virus 1 Bovine Viral Pestivirus Quebec virus diseases General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Intestines Restriction enzyme Restriction site biology.protein Bovine Virus Diarrhea-Mucosal Disease Cattle Spleen |
| Zdroj: | Archives of Virology. 140:1285-1290 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/bf01322754 |
| Popis: | The 5' untranslated region (UTR) of several bovine viral diarrhea virus (BVDV) isolates from the severe Quebec outbreak was amplified by polymerase chain reaction (PCR) and sequenced. Sequences revealed the loss, for the BVDV type II isolates, of an internal PstI restriction site, which is present in all known BVDV type 1 5' UTR sequences. A single restriction enzyme digestion (PstI) of an aliquot of PCR product allowed us to differentiate BVDV type I and BVDV type II. |
| Databáze: | OpenAIRE |
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