Chromosomal localization of 18S rDNA and telomere sequence in the aye-aye, Daubentonia madagascariensis
| Autor: | Yasuhiro Go, Gilbert Rakotoarisoa, Hirohisa Hirai, Naoki Koyama, Roger Mora, Yoshi Kawamoto, Albert Randrianjafy, Yuriko Hirai, Taizo Shima |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Silver Staining Transcription Genetic Biology DNA Ribosomal Chromosomes Evolution Molecular Centromere Nucleolus Organizer Region RNA Ribosomal 18S Genetics medicine Animals Humans Molecular Biology Gene Ribosomal DNA In Situ Hybridization Fluorescence medicine.diagnostic_test Lemur Chromosome Mapping Chromosome Karyotype General Medicine Telomere Ribosomal RNA Karyotyping Female Fluorescence in situ hybridization |
| Zdroj: | Genes & Genetic Systems. 75:299-303 |
| ISSN: | 1880-5779 1341-7568 |
| DOI: | 10.1266/ggs.75.299 |
| Popis: | Chromosomal localization of 18S rDNA and telomere sequence was attempted on the chromosomes of the aye-aye (2n = 30) using fluorescence in situ hybridization (FISH) and primed in situ labeling (PRINS), respectively. The rDNA was localized at the tip or whole of the short arm of acrocentric chromosomes 13 and 14 in all spreads observed. However, post-FISH silver-nitrate (Ag) staining showed that transcriptional activity of the rRNA genes was variable, particularly in chromosome 14, which was most frequently negative in one homologue carrying the smaller copy number of rDNA. This observation supports, at the molecular cytogenetic level, previous data concerning the relationship between the copy number of rDNA and its trancriptional activity. On the other hand, telomere sequence was localized only at the telomeric region of all chromosomes, the so-called telomere-only pattern, a characteristic similar to that of the greater bushbaby. These data may provide information on the chromosomal evolution of the lemur, because locations of rDNA and telomere sequences frequently offer important clues in reconstruction of karyotype differentiation. |
| Databáze: | OpenAIRE |
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