Unidirectional heterologous receptor desensitization between both the fMLP and C5a receptor and the IL-8 receptor
| Autor: | K T Hartiala, R. C. Brower, E E Kwoh, A T Transue, R A Blackwood |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Neutrophils medicine.medical_treatment Immunology Heterologous Complement C5a Biology In Vitro Techniques Calcium in biology C5a receptor Receptors Interleukin-8A Antigens CD Homologous desensitization Internal medicine medicine Immunology and Allergy Humans Receptor Receptor Anaphylatoxin C5a Desensitization (medicine) Interleukin-8 hemic and immune systems Chemotaxis Cell Biology Receptors Interleukin Receptors Complement N-Formylmethionine Leucyl-Phenylalanine Chemotaxis Leukocyte Kinetics Endocrinology Calcium Cell activation |
| Zdroj: | Journal of leukocyte biology. 60(1) |
| ISSN: | 0741-5400 |
| Popis: | During inflammation neutrophils receive multiple signals that are integrated, allowing a single modified response. One mechanism for this discrimination is receptor desensitization, a process whereby ligand-receptor binding is disassociated from cell activation. We examined the effect of heterologous receptor desensitization on neutrophil Chemotaxis, calcium mobilization, and arachidonic acid production, using interleukin-8 (IL-8), C5a, and N-formyl-methionyl-leucyl-phenylalanine (fMLP). We observed reciprocal inhibition with respect to Chemotaxis. We demonstrated that homologous desensitization, with respect to the mobilization of intracellular calcium stores, lasted approximately 15 min. Heterologous desensitization between the fMLP receptor and the C5a receptor was reciprocal; either stimulant would diminish the cells9 response to stimulation by the other for approximately 3–5 min. However, we observed a unidirectional heterologous desensitization of the IL-8 receptor by both the fMLP and the C5a receptor. This unidirectional heterologous desensitization was observed with respect to both calcium mobilization and arachidonic acid production (i.e., prestimulation of the IL-8 receptor had no effect on subsequent stimulation by either fMLP or C5a). |
| Databáze: | OpenAIRE |
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