Specific association of the gene product of PKD2 with the TRPC1 channel
| Autor: | Emily Kim, Vikas P. Sukhatme, Gerd Walz, Thierry Arnould, Leonidas Tsiokas, Chenwen Zhu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
TRPP Cation Channels
Recombinant Fusion Proteins Molecular Sequence Data TRPP Biology Kidney Transfection urologic and male genital diseases TRPC5 Ion Channels Cell Line TRPC1 Humans Amino Acid Sequence education Integral membrane protein TRPC TRPC Cation Channels education.field_of_study Binding Sites Multidisciplinary Sequence Homology Amino Acid urogenital system Membrane Proteins Proteins Biological Sciences Polycystic Kidney Autosomal Dominant Recombinant Proteins female genital diseases and pregnancy complications Cell biology Polycystin 2 Membrane protein Biochemistry Calcium Channels Sequence Alignment |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 96(7):3934-3939 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | The function(s) of the genes ( PKD1 and PKD 2) responsible for the majority of cases of autosomal dominant polycystic kidney disease is unknown. While PKD1 encodes a large integral membrane protein containing several structural motifs found in known proteins involved in cell–cell or cell–matrix interactions, PKD2 has homology to PKD1 and the major subunit of the voltage-activated Ca 2+ channels. We now describe sequence homology between PKD2 and various members of the mammalian transient receptor potential channel (TRPC) proteins, thought to be activated by G protein-coupled receptor activation and/or depletion of internal Ca 2+ stores. We show that PKD2 can directly associate with TRPC1 but not TRPC3 in transfected cells and in vitro . This association is mediated by two distinct domains in PKD2. One domain involves a minimal region of 73 amino acids in the C-terminal cytoplasmic tail of PKD2 shown previously to constitute an interacting domain with PKD1. However, distinct residues within this region mediate specific interactions with TRPC1 or PKD1. The C-terminal domain is sufficient but not necessary for the PKD2–TRPC1 association. A more N-terminal domain located within transmembrane segments S2 and S5, including a putative pore helical region between S5 and S6, is also responsible for the association. Given the ability of the TRPC to form functional homo- and heteromultimeric complexes, these data provide evidence that PKD2 may be functionally related to TRPC proteins and suggest a possible role of PKD2 in modulating Ca 2+ entry in response to G protein-coupled receptor activation and/or store depletion. |
| Databáze: | OpenAIRE |
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