Glucose Uptake and Metabolism in grr1/cat80 Mutants of Sacharomyces cerevisiae
| Autor: | Andrea Weber, Michael Ciriacy, Kerstin Freidel, Sabire Özcan, Frank Schulte |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Lactose permease
Genes Viral Monosaccharide Transport Proteins Glucose uptake Restriction Mapping Saccharomyces cerevisiae Mutant Gene Expression Biochemistry chemistry.chemical_compound DNA Fungal Maltose biology Genetic Complementation Test Membrane Transport Proteins Biological Transport RNA Fungal Metabolism biology.organism_classification Complementation Kinetics Glucose chemistry Flux (metabolism) Plasmids |
| Zdroj: | European Journal of Biochemistry. 224:605-611 |
| ISSN: | 1432-1033 0014-2956 |
| Popis: | Glucose repression in the yeast Saccharomyces cerevisiae designates a global regulatory system controlling the expression of various sets of genes required for the utilization of alternate carbon sources. In a screen, designed for the selection of mutants with reduced glycolytic flux we obtained isolates which were shown by complementation of the cloned wild-type gene to be allelic to the glucose repression mutants grr1/cat80/cot2 previously described. We demonstrate that the grr1 lesion lead to a concentration-dependent decrease in glycolytic flux on glucose. It is very likely that this is caused by a significant decrease in the expression of various genes encoding hexose transporters (HXT1,3) leading to a reduced glucose-uptake rate. In contrast, expression of the maltose permease gene (MAL11) and maltose utilization is normal. There is indirect evidence that grr1 affects the uptake of amino acids, and others have shown that the sugar-induced transport of divalent cations is impaired. These effects are not glucose-specific. We suggest that Grr1, a putative cytoplasmic protein, has a central function in the sensing of nutritional conditions for a variety of unrelated substances, and that relief from glucose repression may be a corollary of this defect in sensing. |
| Databáze: | OpenAIRE |
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