The effect of ICRT‐3 on Wnt signaling pathway in head and neck cancer
| Autor: | Aycan Asik, Besra Ozmen Yelken, Cagla Kayabasi, Cigir Biray Avci, Fatma Dogan, Cumhur Gündüz, Fatma Sogutlu, Sunde Yilmaz Susluer, Roya Gasimli |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell cycle checkpoint Cell Survival Lymphoid Enhancer-Binding Factor 1 Apoptosis Biochemistry Inhibitory Concentration 50 03 medical and health sciences Transcription Factor 4 0302 clinical medicine Cell Movement Cell Line Tumor Coactivator Humans Viability assay Cytotoxicity Oxazoles Wnt Signaling Pathway Molecular Biology beta Catenin Hypopharyngeal Neoplasms Caspase 3 Cytotoxins Chemistry Wnt signaling pathway Cell Cycle Checkpoints Cell Biology Cell cycle Caspase 9 Gene Expression Regulation Neoplastic 030104 developmental biology Head and Neck Neoplasms 030220 oncology & carcinogenesis Carcinoma Squamous Cell Neoplastic Stem Cells Cancer research Stem cell Signal Transduction |
| Zdroj: | Journal of Cellular Biochemistry. 120:380-395 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.27393 |
| Popis: | The effect of Wnt pathway in head and neck cancer could not be elucidated, even though the aberrant Wnt signaling plays a key role in the development of many types of cancer. The inhibitor of β-catenin responsive transcription (ICRT-3) blocks the Wnt signaling pathway by binding to β-catenin, which is a coactivator of the Wnt signaling pathway and a promising agent for inhibiting aberrant signaling. In our study, we aimed to evaluate the effect of ICRT-3 on the cytotoxicity, apoptosis, cell cycle progression, migration, and gene expressions in head and neck cancer stem cell (HNCSC) and hypopharynx cancer. The effect of this compound on cytotoxicity and cell viability in FaDu and HNCSC line was assessed by using the water-soluble tetrazolium salt-1 method. The effect of ICRT-3 on apoptosis was detected by using Annexin V and caspase-3, caspase-9 kit, on cell cycle progression by cycle test plus DNA reagent kit, on gene expression by dual luciferase reporter assay, and on migration activity by wound healing assay in both cell lines. ICRT-3 was determined to have cytotoxic and apoptotic effect in both cell lines. In addition, it was also found that the administration of ICRT-3 caused cell cycle arrest and significant decrease in gene expression level and migration ability of the cells. |
| Databáze: | OpenAIRE |
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