Single-cell sequencing reveals the origin and the order of mutation acquisition in T-cell acute lymphoblastic leukemia
| Autor: | Lucienne Michaux, Ellen Geerdens, Jolien De Bie, Nancy Boeckx, Kim De Keersmaecker, Heidi Segers, Jan Cools, Sofie Demeyer, Peter Vandenberghe, Llucia Alberti-Servera, Michaël Broux, Anne Uyttebroeck, Thierry Voet |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Myeloid T cell CD34 BIOLOGY CLONAL ORIGINS Antigens CD34 Biology RELAPSE medicine.disease_cause Precursor T-Cell Lymphoblastic Leukemia-Lymphoma Article 03 medical and health sciences 0302 clinical medicine OF-FUNCTION MUTATIONS medicine Humans Progenitor cell Receptor Notch1 Mutation Science & Technology Whole Genome Sequencing TRANSPLANTATION Gene Expression Profiling Multipotent Stem Cells Hematology medicine.disease CANCER ADP-ribosyl Cyclase 1 EVOLUTION 3. Good health Leukemia 030104 developmental biology medicine.anatomical_structure Oncology Single cell sequencing 030220 oncology & carcinogenesis Cancer research Bone marrow Life Sciences & Biomedicine GENOMICS |
| Zdroj: | Leukemia |
| ISSN: | 1476-5551 |
| Popis: | Next-generation sequencing has provided a detailed overview of the various genomic lesions implicated in the pathogenesis of T-cell acute lymphoblastic leukemia (T-ALL). Typically, 10-20 protein-altering lesions are found in T-ALL cells at diagnosis. However, it is currently unclear in which order these mutations are acquired and in which progenitor cells this is initiated. To address these questions, we used targeted single-cell sequencing of total bone marrow cells and CD34+CD38- multipotent progenitor cells for four T-ALL cases. Hierarchical clustering detected a dominant leukemia cluster at diagnosis, accompanied by a few smaller clusters harboring only a fraction of the mutations. We developed a graph-based algorithm to determine the order of mutation acquisition. Two of the four patients had an early event in a known oncogene (MED12, STAT5B) among various pre-leukemic events. Intermediate events included loss of 9p21 (CDKN2A/B) and acquisition of fusion genes, while NOTCH1 mutations were typically late events. Analysis of CD34+CD38- cells and myeloid progenitors revealed that in half of the cases somatic mutations were detectable in multipotent progenitor cells. We demonstrate that targeted single-cell sequencing can elucidate the order of mutation acquisition in T-ALL and that T-ALL development can start in a multipotent progenitor cell. ispartof: LEUKEMIA vol:32 issue:6 pages:1358-1369 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink