Predictive value of tissue calprotectin for disease recurrence after ileocecal resection in pediatric Crohn's disease
| Autor: | Filip Mikus, Lucie Pos, Kristyna Zarubova, Vojtech Dotlacil, Richard Skaba, Ondrej Hradsky, Tereza Lerchova, Ondrej Fabian, Jiri Bronsky |
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| Rok vydání: | 2022 |
| Předmět: |
medicine.medical_specialty
Peritonitis Disease Anastomosis Gastroenterology General Biochemistry Genetics and Molecular Biology Feces fluids and secretions Crohn Disease Recurrence Internal medicine medicine Humans Child business.industry Margins of Excision Colonoscopy Ileocecal resection medicine.disease Predictive value Resection margin Immunohistochemistry Calprotectin business Leukocyte L1 Antigen Complex Biomarkers |
| Zdroj: | Biomedical Papers. 166:297-303 |
| ISSN: | 1804-7521 1213-8118 |
| Popis: | AIM Detection of possible predictive factors of endoscopic recurrence after ileocecal resection in Crohn's disease could be very beneficial for the individual adjustment of postoperative therapy. The aim of this study was to verify, whether immunohistochemical detection of calprotectin in resection margins is useful in diagnostics of endoscopic recurrence. METHODS In this study we included pediatric patients with Crohn's disease who underwent ileocecal resection, regardless of pre-operative or post-operative therapy (n=48). We collected laboratory, clinical, surgical, endoscopic and histopathological data at the time of surgery and at 6 months after surgery. The immunohistochemical staining of calprotectin antigen was performed on all paraffin blocks from the resection margins. RESULTS Out of 48 patients 52% had endoscopic recurrence in the anastomosis (defined by Rutgeerts score) within 6 months after surgery. The number of cells positive for calprotectin in the proximal resection margin was negatively associated with recurrence (P=0.008), as was the elevated level of total calprotectin (from both resection margins). There was no correlation of calprotectin in distal resection margin and endoscopic recurrence. Fecal calprotectin over 100 ug/g (P=0.0005) and high CRP (P |
| Databáze: | OpenAIRE |
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