Further characterization of a melanoma-specific protein from human urine
| Autor: | K.B. Cooke, Claire Bennett |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Cancer Research
Sialoglycoproteins Alpha (ethology) complex mixtures Chromatography Affinity Carcinoembryonic antigen Antibody Specificity Antigens Neoplasm parasitic diseases Humans Melanoma neoplasms Polyacrylamide gel electrophoresis Antiserum chemistry.chemical_classification biology Isoelectric focusing Molecular biology Carcinoembryonic Antigen Neoplasm Proteins Molecular Weight Oncology chemistry biology.protein Electrophoresis Polyacrylamide Gel alpha-Fetoproteins Ultracentrifuge Isoelectric Focusing Melanoma-Specific Antigens Glycoprotein Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.1980.135 |
| Popis: | Isolation of a melanoma-specific protein (MSP) from human urine has been achieved using antibody affinity chromatography. MSP migrates as a single homogeneous protein on SDS PAGE and comparison of these data and ultracentrifuge analyses indicates that MSP contains a single polypeptide chain. MSP, however, shows considerable charge heterogeneity on isoelectric focusing. The desialo form, alpha 2 MSP, is found predominantly in patients with advanced metastatic disease, whilst only the sialo form alpha 1 MSP, is obtained from the urine of patients with early-stage disease. MSP does not react with antisera raised to alpha 1 foetoprotein (AFP) or carcinoembryonic antigen (CEA) and hence is immunologically distinct from these other tumour-associated glycoproteins. Antisera raised to MSP do not react with normal skin melanocytes nor with any foetal tissue tested, and hence the origin of MSP remains unresolved. Images Fig. 1 Fig. 2 |
| Databáze: | OpenAIRE |
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