Allele-specific real-time PCR testing for minor macrolide-resistant Mycoplasma Pneumoniae

Autor:	Dan Li, Wenjuan Hu, Zhaoyong Wu, Dongxing Guo, Baoping Xu, Xiujun Tian, Jingyi Li, Kunling Shen, Shaogang Li, Ran Wei, Deli Xin
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0301 basic medicine DNA Bacterial Mycoplasma pneumoniae 030106 microbiology Population Viral quasispecies Biology medicine.disease_cause Real-Time Polymerase Chain Reaction A2063G lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine 23S ribosomal RNA Drug Resistance Bacterial Pneumonia Mycoplasma medicine Humans Point Mutation lcsh:RC109-216 030212 general & internal medicine education education.field_of_study Point mutation Reproducibility of Results 23S rRNA A2064G Virology Allele-specific real-time PCR Anti-Bacterial Agents RNA Ribosomal 23S Infectious Diseases Real-time polymerase chain reaction Parasitology Pharynx Female Macrolides Nested polymerase chain reaction Research Article
Zdroj:	BMC Infectious Diseases, Vol 19, Iss 1, Pp 1-9 (2019) BMC Infectious Diseases
ISSN:	1471-2334
Popis:	Background The point mutations in 23S rRNA gene of Mycoplasma pneumoniae (M. pneumoniae) can lead to high-level resistance to macrolides. This study aimed to evaluate allele-specific real-time PCR (ASPCR) to detect the resistance-related mutations located at positions A2063G and A2064G of 23S rRNA gene. Methods We detected 178 pharyngeal swab specimens and calculated the proportions of resistant and sensitive quasispecies using ASPCR assays. ASPCR assays can detect down to 10 copies of 23S rRNA gene and achieved sensitivities of
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0454690c545785e6f5ff68f7e37d6057 http://link.springer.com/article/10.1186/s12879-019-4228-4 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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