Measurement of cholecystokinin in plasma with reference to nutrition related obesity studies
| Autor: | Jens F. Rehfeld |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Biomedical Research Nutritional Sciences Endocrinology Diabetes and Metabolism Nutritional Status 030209 endocrinology & metabolism Satiety Response digestive system Plasma 03 medical and health sciences 0302 clinical medicine Endocrinology Bias Internal medicine Gastrins medicine Animals Humans Obesity Gastrin Cholecystokinin High concentration 030109 nutrition & dietetics Nutrition and Dietetics Appetite Regulation business.industry digestive oral and skin physiology Blood Proteins medicine.disease Peptide Fragments business Blood Chemical Analysis hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Nutrition Research. 76:1-8 |
| ISSN: | 0271-5317 |
| DOI: | 10.1016/j.nutres.2020.01.003 |
| Popis: | This review describes the premises for accurate measurement of the gut hormone and satiety factor cholecystokinin (CCK) in circulation. Such a description is useful for nutrition and obesity research in which CCK in its satiety role has evoked considerable interest during the last decades. The background for the review is two sorts of considerations or concerns. First, CCK is a complex peptide system that in several ways challenges plasma measurements because the concentrations in plasma are very low (in the femtomolar to low picomolar range), and the bioactive CCK circulates in different molecular forms (CCK-58, -33, -22, and -8). Furthermore, there are major specificity problems because the structurally similar gastrin hormone circulates in 10- to 20-fold higher concentrations, and in addition, plasma proteins may, due to their high concentration, interfere in an unspecific way with immunoassay measurements. The second concern is that several obesity studies in recent decades have been based on commercial CCK kits with often inadequate documentation of the reliability in plasma measurement. Consequently, many plasma CCK results in today's obesity studies are difficult to compare. Moreover, the use of even fairly reliable commercial CCK kits has recently suffered from sudden discontinuation of the kit production, which has endangered several projects in nutrition and obesity research. |
| Databáze: | OpenAIRE |
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