| Autor: |
Mengdan Li, Banjun Ruan, Wei, Jing, Yang, Qi, Mingwei Chen, Meiju Ji, Hou, Peng |
| Rok vydání: |
2020 |
| DOI: |
10.6084/m9.figshare.12457358.v1 |
| Popis: |
Additional file 2: Figure S1. ACYP2 knockdown in glioma cells were confirmed by qRT-PCR assay. Figure S2. ACYP2 knockdown inhibits migration and invasion abilities of glioma cells. Figure S3. Ectopic expression of ACYP2 in SHG44 and A172 cells promoted cell migration and invasion compared to the control. Figure S4. The effect of ACYP2 knockdown on Ca2+ levels in endoplasmic reticulum (ER) in glioma cells. Figure S5. BAPTA-AM treatment reverses inhibitory effect of ACYP2 depletion on glioma cell migration. Figure S6. Calpeptin treatment reverses inhibitory effect of ACYP2 depletion on glioma cell migration. Figure S7. The effect of ACYP2 knockdown on the activity of NFATc1 in glioma cells. Figure S8. qRT-PCR was used to determine the effect of ACYP2 knockdown on the expression of NF-kB’s downstream targets (Bcl-xL and Bcl-2) in the indicated cells. Figure S9. qRT-PCR was used to determine the effect of ACYP2 knockdown on the expression of c-Myc’s downstream targets (E2F2 and cyclin E) and p-STAT3’s downstream targets (c-Fos and c-Jun). Figure S10. Cells transfected with the indicated constructs were treated with the vehicle or Stattic, and the MTT assay was then carried out to evaluate their effect on cell proliferation. Figure S11. qRT-PCR assay was performed to determine mRNA expression levels of PMCA1–4 in the indicated glioma cell lines. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
