Probing in Vitro Ribose Induced DNA-Glycation Using Raman Microspectroscopy
| Autor: | Christophe Eklouh-Molinier, Laurence Van-Gulick, Michael Fere, Goutam Kumar Chandra, Pierre Jeannesson, Jean-François Angiboust, Cyril Gobinet, Olivier Piot |
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| Přispěvatelé: | Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
Glycosylation
Ribose In Vitro Techniques Spectrum Analysis Raman 010402 general chemistry 01 natural sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound symbols.namesake Glycation Animals 030304 developmental biology 0303 health sciences Chemistry DNA In vitro 0104 chemical sciences Raman microspectroscopy Biochemistry [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic symbols Nucleic Acid Conformation Cattle Indicators and Reagents Spectrophotometry Ultraviolet Raman spectroscopy Two-dimensional nuclear magnetic resonance spectroscopy Long term control |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2015, 87, pp.2655-2664. ⟨10.1021/acs.analchem.5b00182⟩ |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.5b00182 |
| Popis: | International audience; To identify and characterize glycation, induced modifications of DNA are crucial toward understanding their functional significance due to their significant role in the long term control of aging and age-related diseases. In this study, we present the ability of Raman microspectroscopy as a novel analytical technique for a rapid and reliable identification of glycated DNA in a reagent-free manner. We have demonstrated that this technique has potential to provide very small conformational modifications. The combination of principal component analysis (PCA) and two-dimensional (2D) correlation spectroscopy has assisted us to explore in vitro DNA-glycation and provide more insights into the dynamics of the DNAglycation process in an easier fashion. PCA analysis of Raman spectra shows a clear discrimination between native and glycated DNA samples. On the other hand, 2D correlation Raman analysis provides sequential order of the mechanism of the DNA-glycation process, and most likely, it occurs in the following sequence: Structural modifications of individual nucleobases (G > A > C) → DNA backbone modifications → partial transition of DNA conformations (A to B form). Our observations clearly suggest that the structure of DNA is altered, i.e., a partial transition of DNA backbone conformation from A to B form when glycated, but does not induce any final transition in DNA double helix conformation, and eventually, DNA presents in an intermediate A−B form, more toward the B form. |
| Databáze: | OpenAIRE |
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