Polyhydroxyalkanoate as a slow-release carbon source for in situ bioremediation of contaminated aquifers: From laboratory investigation to pilot-scale testing in the field
| Autor: | Simona Rossetti, Lucia Pierro, Ernst Bartsch, Firoozeh Arjmand, Salvatore Sucato, Marco Petrangeli Papini, Eduard Alesi, Bruna Matturro, Marco Sagliaschi |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
PHB
Polyesters 0208 environmental biotechnology Hydroxybutyrates Bioengineering Aquifer Electron donor Pilot Projects 02 engineering and technology 010501 environmental sciences 01 natural sciences Water Purification Biological reductive dechlorination Chlorinated aliphatic hydrocarbons chemistry.chemical_compound Groundwater circulation well Bioremediation Bioreactors Groundwater pollution Reductive dechlorination Hydrocarbons Chlorinated hydrocarbons Molecular Biology Groundwater 0105 earth and related environmental sciences geography aquifer geography.geographical_feature_category Chemistry Polyhydroxyalkanoates General Medicine Chloroflexi Carbon 020801 environmental engineering Biodegradation Environmental Italy Environmental chemistry Fermentation Poly (3-hydroxybutyrate) Water treatment Water Pollutants Chemical Water well Biotechnology Real-time PCR |
| Zdroj: | New biotechnology 37 (2017): 60–68. doi:10.1016/j.nbt.2016.11.004 info:cnr-pdr/source/autori:Pierro, Lucia; Matturro, Bruna; Rossetti, Simona; Sagliaschi, Marco; Sucato, Salvatore; Alesi, Editard; Bartsch, Ernst; Arjmand, Firoozeh; Petrangeli Papini, Marco/titolo:Polyhydroxyalkanoate as a slow-release carbon source for in situ bioremediation of contaminated aquifers: From laboratory investigation to pilot-scale testing in the field/doi:10.1016%2Fj.nbt.2016.11.004/rivista:New biotechnology (Print)/anno:2017/pagina_da:60/pagina_a:68/intervallo_pagine:60–68/volume:37 New biotechnology (Online) Volume 37 (2017). doi:10.1016/j.nbt.2016.11.004 info:cnr-pdr/source/autori:Pierro L, Matturro B, Rossetti S, Sagliaschi M, Sucato S, Alesi E, Bartsch E, Arjmand F, Papini MP/titolo:Polyhydroxyalkanoate as a slow-release carbon source for in situ bioremediation of contaminated aquifers: From laboratory investigation to pilot-scale testing in the field./doi:10.1016%2Fj.nbt.2016.11.004/rivista:New biotechnology (Online)/anno:2017/pagina_da:/pagina_a:/intervallo_pagine:/volume:Volume 37 |
| Popis: | A pilot-scale study aiming to evaluate the potential use of poly-3-hydroxy-butyrate (PHB) as an electron donor source for in situ bioremediation of chlorinated hydrocarbons in groundwater was conducted. Compared with commercially available electron donors, PHB offers a restricted fermentation pathway (i.e., through acetic acid and molecular hydrogen) by avoiding the formation of any residual carbon that could potentially spoil groundwater quality. The pilot study was carried out at an industrial site in Italy, heavily contaminated by different chlorinated aliphatic hydrocarbons (CAHs). Prior to field testing, PHB was experimentally verified as a suitable electron donor for biological reductive dechlorination processes at the investigated site by microcosm studies carried out on site aquifer material and measuring the quantitative transformation of detected CAHs to ethene. Owing to the complex geological characteristics of the aquifer, the use of a groundwater circulation well (GCW) was identified as a potential strategy to enable effective delivery and distribution of electron donors in less permeable layers and to mobilise contaminants. A 3-screened, 30-m-deep GCW coupled with an external treatment unit was installed at the site. The effect of PHB fermentation products on the in situ reductive dechlorination processes were evaluated by quantitative real-time polymerase chain reaction (qPCR). The results from the first 4 months of operation clearly demonstrated that the PHB fermentation products were effectively delivered to the aquifer and positively influenced the biological dechlorination activity. Indeed, an increased abundance of Dehalococcoides mccartyi (up to 6.6 fold) and reduced CAH concentrations at the installed monitoring wells were observed. (C) |
| Databáze: | OpenAIRE |
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