Reappraisal of Vipera aspis venom neurotoxicity
| Autor: | Isabelle Guillemin, Sabine Jourdain, Valérie Choumet, Alexandre Teynié, Elisabeth Ferquel, Jacques d'Alayer, Virginie Jan, Luc de Haro |
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| Přispěvatelé: | Venins, Institut Pasteur [Paris], Centre antipoison et de toxicovigilance (Marseille) (CAPTV Marseille), Assistance Publique - Hôpitaux de Marseille (APHM), Ciphergen, Unité Expérimentale de Nutrition Comparée (UENC), Institut National de la Recherche Agronomique (INRA), Analyse et Microséquençage des Protéines (Plate-forme), Protéomique (Plate-Forme), Centre antipoison, hopital salvador, Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Messenger
lcsh:Medicine Venom MESH: Base Sequence Mass Spectrometry MESH: Reverse Transcriptase Polymerase Chain Reaction MESH: Animals lcsh:Science MESH: Phospholipases A2 0303 health sciences education.field_of_study Multidisciplinary biology Reverse Transcriptase Polymerase Chain Reaction 030302 biochemistry & molecular biology MESH: Enzyme-Linked Immunosorbent Assay Anatomy people.cause_of_death 3. Good health Biochemistry/Molecular Evolution Venomous snake Snake venom Biotechnology/Protein Chemistry and Proteomics MESH: Viper Venoms Biochemistry/Transcription and Translation Research Article Autre (Sciences du Vivant) MESH: DNA Primers [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] Population Neurotoxins Public Health and Epidemiology Zoology Enzyme-Linked Immunosorbent Assay Viper Venoms Neurological Disorders complex mixtures 03 medical and health sciences medicine Animals RNA Messenger Envenomation education Vipera aspis 030304 developmental biology DNA Primers MESH: Neurotoxins MESH: RNA Messenger Base Sequence Phospholipases A2 RNA MESH: Mass Spectrometry lcsh:R biology.organism_classification medicine.disease Snake bites Vipera lcsh:Q people |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2007, 2 (11), pp.e1194. ⟨10.1371/journal.pone.0001194⟩ Plos One 11 (2), e1194. (2007) PLoS ONE, Vol 2, Iss 11, p e1194 (2007) PLoS ONE, 2007, 2 (11), pp.e1194. ⟨10.1371/journal.pone.0001194⟩ |
| ISSN: | 1932-6203 |
| Popis: | International audience; BACKGROUND: The variation of venom composition with geography is an important aspect of intraspecific variability in the Vipera genus, although causes of this variability remain unclear. The diversity of snake venom is important both for our understanding of venomous snake evolution and for the preparation of relevant antivenoms to treat envenomations. A geographic intraspecific variation in snake venom composition was recently reported for Vipera aspis aspis venom in France. Since 1992, cases of human envenomation after Vipera aspis aspis bites in south-east France involving unexpected neurological signs were regularly reported. The presence of genes encoding PLA(2) neurotoxins in the Vaa snake genome led us to investigate any neurological symptom associated with snake bites in other regions of France and in neighboring countries. In parallel, we used several approaches to characterize the venom PLA(2) composition of the snakes captured in the same areas. METHODOLOGY/PRINCIPAL FINDINGS: We conducted an epidemiological survey of snake bites in various regions of France. In parallel, we carried out the analysis of the genes and the transcripts encoding venom PLA(2)s. We used SELDI technology to study the diversity of PLA(2) in various venom samples. Neurological signs (mainly cranial nerve disturbances) were reported after snake bites in three regions of France: Languedoc-Roussillon, Midi-Pyrénées and Provence-Alpes-Côte d'Azur. Genomes of Vipera aspis snakes from south-east France were shown to contain ammodytoxin isoforms never described in the genome of Vipera aspis from other French regions. Surprisingly, transcripts encoding venom neurotoxic PLA(2)s were found in snakes of Massif Central region. Accordingly, SELDI analysis of PLA(2) venom composition confirmed the existence of population of neurotoxic Vipera aspis snakes in the west part of the Massif Central mountains. CONCLUSIONS/SIGNIFICANCE: The association of epidemiological studies to genetic, biochemical and immunochemical analyses of snake venoms allowed a good evaluation of the potential neurotoxicity of snake bites. A correlation was found between the expression of neurological symptoms in humans and the intensity of the cross-reaction of venoms with anti-ammodytoxin antibodies, which is correlated with the level of neurotoxin (vaspin and/or ammodytoxin) expression in the venom. The origin of the two recently identified neurotoxic snake populations is discussed according to venom PLA(2) genome and transcriptome data. |
| Databáze: | OpenAIRE |
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