iNOS/Arginase-1 expression in the pulmonary tissue over time during Cryptococcus gattii infection
| Autor: | Patrícia Kellen Martins Oliveira-Brito, Fausto Almeida, Caroline Patini Rezende, Maria Cristina Roque-Barreira, Thiago Aparecido da Silva |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
biology Inhalation CRIPTOCOCOSE Immunology Cell Biology biology.organism_classification medicine.disease Microbiology Arginase 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Infectious Diseases Immune system 030220 oncology & carcinogenesis Cryptococcus gattii infection Cryptococcosis medicine Molecular Biology Cryptococcus gattii |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1753-4267 1753-4259 |
| DOI: | 10.1177/1753425919869436 |
| Popis: | Inhalation of Cryptococcus gattii yeasts (causing cryptococcosis) triggers an anti-cryptococcal immune response initiated by macrophages, neutrophils or dendritic cells, and the iNOS expressed by various cells may regulate the function and differentiation of innate and adaptive immune cells. Here, we evaluated the effect of progression of C. gattii infection on the host innate immune response. C. gattii infection in BALB/c mice spreads to several organs by 21 d post infection. The numbers of neutrophils and lymphocytes in the peripheral blood of C. gattii–infected mice were remarkably altered on that day. The frequency of CD11b+ cells and cell concentrations of CD4+ and CD8+ T cells was significantly altered in the pulmonary tissue of infected mice. We found a higher frequency of CD11b+/iNOS+ cells in the lungs of infected mice, accompanied by an increase in frequency of CD11b+/Arginase-1+ cells over time. Moreover, the iNOS/Arginase-1 expression ratio in CD11b+ cells reached its lowest value at 21 d post infection. In addition, the cytokine micro-environment in infected lungs did not show a pro-inflammatory profile. Surprisingly, iNOS knock-out prolonged the survival of infected mice, while their pulmonary fungal burden was higher than that of infected WT mice. Thus, C. gattii infection alters the immune response in the pulmonary tissue, and iNOS expression may play a key role in infection progression. |
| Databáze: | OpenAIRE |
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