Polymorphisms of transporter associated with antigen processing genes in atopic dermatitis
| Autor: | Hidemi Nakagawa, Shoji Kuwata, Takafumi Etoh, Masami Yanagisawa, Takeo Juji, Katsushi Tokunaga, Hidehisa Saeki, Yoichi Shibata |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Adolescent Immunology Molecular Sequence Data Human leukocyte antigen Biology Polymerase Chain Reaction Dermatitis Atopic ATP Binding Cassette Transporter Subfamily B Member 3 HLA Antigens HLA-DQ Antigens medicine Odds Ratio Immunology and Allergy Humans Allele ATP Binding Cassette Transporter Subfamily B Member 2 Child Allele frequency Gene Alleles Genetics Chi-Square Distribution Polymorphism Genetic Base Sequence Haplotype Atopic dermatitis HLA-DR Antigens medicine.disease Molecular biology Haplotypes biology.protein TAP2 ATP-Binding Cassette Transporters Female TAP1 Carrier Proteins Polymorphism Restriction Fragment Length |
| Zdroj: | The Journal of allergy and clinical immunology. 94(3 Pt 2) |
| ISSN: | 0091-6749 |
| Popis: | We investigated polymorphisms of transporter associated with antigen process (TAP) genes in atopic dermatitis. We developed a polymerase chain reaction–restriction fragment length polymorphism method for discriminating TAP alleles. Genomic DNA was obtained from 29 Japanese patients with atopic dermatitis and 35 control subjects. Dimorphic regions of TAP1 and TAP2 genes were amplified by polymerase chain reaction. Amplified products were digested with restriction endonucleases to determine TAP alleles: Sau3A1 for TAP1 codon 333 (Ile-Val), AccI for TAP1 codon 637 (Asp-Gly), and EcoRII for TAP2 codon 687 (Gln-Stop). We observed four alleles for TAP1 and dimorphism for TAP2 codon 687. Six of 35 controls had the TAP1 D allele, which has been reported to be a rare allele in Caucasian populations. Gene frequency of TAP1 637Asp exhibited a tendency to increase in the patients with atopic dermatitis. TAP1 637Asp and TAP1 A alleles were estimated to constitute a haplotype with DRB1*1302-DQB1*0604 and DRB1*0803-DQB1*0601 in the Japanese population. Because TAP1 and TAP2 genes are located between HLA-DQB1 and -DPB1 loci, analysis of TAP gene polymorphisms will be useful for a better understanding of susceptibility loci in HLA class II–associated disease. (J ALLERGY CLIN IMMUNOL 1994;94:565-74.) |
| Databáze: | OpenAIRE |
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