Quantifying the effect of PEG architecture on nanoparticle ligand availability using DNA-PAINT†
| Autor: | Silvia Pujals, Teodora Andrian, Lorenzo Albertazzi |
|---|---|
| Přispěvatelé: | ICMS Core, Nanoscopy for Nanomedicine, Molecular Biosensing for Med. Diagnostics |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
SURFACE Nanoparticle Bioengineering Nanotechnology 02 engineering and technology macromolecular substances 010402 general chemistry 01 natural sciences chemistry.chemical_compound SYSTEMS PEG ratio Microscopy General Materials Science CHAIN-LENGTH Nanoscopic scale density Nanopartícules Ligand General Engineering Rational design technology industry and agriculture PLGA General Chemistry 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences 3. Good health Microscòpia Chemistry chemistry Particle Nanoparticles 0210 nano-technology Ethylene glycol |
| Zdroj: | Nanoscale Advances Dipòsit Digital de la UB Universidad de Barcelona Nanoscale Advances, 3(24), 6876-6881. Royal Society of Chemistry |
| ISSN: | 2516-0230 |
| Popis: | The importance of PEG architecture on nanoparticle (NP) functionality is known but still difficult to investigate, especially at a single particle level. Here, we apply DNA Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT), a super-resolution microscopy (SRM) technique, to study the surface functionality in poly(lactide-co-glycolide)–poly(ethylene glycol) (PLGA–PEG) NPs with different PEG structures. We demonstrated how the length of the PEG spacer can influence the accessibility of surface chemical functionality, highlighting the importance of SRM techniques to support the rational design of functionalized NPs. The importance of PEG architecture on nanoparticle (NP) functionality is known but still difficult to investigate. Here, DNA-PAINT and qPAINT are used to quantify this phenomenon at a single particle and molecular level. |
| Databáze: | OpenAIRE |
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