Curcumin protects against thioacetamide-induced hepatic fibrosis by attenuating the inflammatory response and inducing apoptosis of damaged hepatocytes
| Autor: | Sheng-Shih Chen, Chih-Hsien Chiu, I-Shu Chen, Shuchen Hsieh, Mu-En Wang, Yi-Chen Chen |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Liver Cirrhosis
Male Curcumin Liver cytology Endocrinology Diabetes and Metabolism Clinical Biochemistry Antineoplastic Agents Apoptosis Inflammation Thioacetamide Biology Biochemistry Antioxidants Cell Line Mice chemistry.chemical_compound Bcl-2-associated X protein Hepatic Stellate Cells In Situ Nick-End Labeling medicine Animals RNA Messenger Molecular Biology Cell Proliferation bcl-2-Associated X Protein Mice Inbred BALB C Nutrition and Dietetics Molecular biology Gene Expression Regulation Liver chemistry Hepatocytes Cancer research biology.protein Hepatic stellate cell Tumor Suppressor Protein p53 medicine.symptom Hepatic fibrosis DNA Damage |
| Zdroj: | The Journal of Nutritional Biochemistry. 23:1352-1366 |
| ISSN: | 0955-2863 |
| Popis: | Inflammation and hepatic stellate cell (HSC) activation are the most crucial steps in the formation of hepatic fibrosis. Hepatocytes damaged by viral or bacterial infection, alcohol or toxic chemicals initiate an inflammatory response that activates collagen production by HSCs. Recent studies indicate curcumin has liver-protective effects due to its anti-inflammatory, antioxidant and anticancer activities; however, the mechanisms are not well understood. In this study, we show that curcumin protected against hepatic fibrosis in BALB/c mice in vivo by inhibiting HSC activation, inflammatory responses and inducing apoptosis of damaged hepatocytes. Using the thioacetamide (TAA)-induced hepatic fibrosis animal model, we found that curcumin treatment up-regulated P53 protein expression and Bax messenger RNA (mRNA) expression and down-regulated Bcl-2 mRNA expression. Together, these responses increased hepatocyte sensitivity to TAA-induced cytotoxicity and forced the damaged cells to undergo apoptosis. Enhancing the tendency of damaged hepatocytes to undergo apoptosis may be the protective mechanism whereby curcumin suppresses inflammatory responses and hepatic fibrogenesis. These results provide a novel insight into the cause of hepatic fibrosis and the cytoprotective effects curcumin has on hepatic fibrosis suppression. |
| Databáze: | OpenAIRE |
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