Interactions of Ionic Liquids and Spirocyclic Compounds with Liposome Model Membranes. A Steady-State Fluorescence Anisotropy Study
| Autor: | Paulus Hyväri, Alistair W. T. King, Antti H. Rantamäki, Gabriel Partl, Jussi Helminen, Susanne K. Wiedmer, Wen Chen |
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| Přispěvatelé: | Department of Chemistry, HUS Head and Neck Center, Susanne Wiedmer / Principal Investigator |
| Rok vydání: | 2019 |
| Předmět: |
DYNAMICS
Lipid Bilayers 116 Chemical sciences lcsh:Medicine Ionic Liquids 02 engineering and technology 01 natural sciences TOXICITY Membrane biophysics chemistry.chemical_compound Biomimetics lcsh:Science Anisotropy Surface-Active Agents/chemistry Liposome Phosphatidylcholines/chemistry Multidisciplinary CHOLESTEROL Bilayer Membrane structure and assembly 021001 nanoscience & nanotechnology Chemical biology Lipids Chemical safety Membrane Cholesterol/chemistry Toxicity Phosphatidylcholines DPH Permeation and transport lipids (amino acids peptides and proteins) Membranes/chemistry 0210 nano-technology Diphenylhexatriene Liposomes/chemistry Lipid Bilayers/chemistry PHYSICAL-PROPERTIES Fluorescence Polarization Phosphatidylserines 010402 general chemistry Article Phosphatidylserines/chemistry PROBES Surface-Active Agents Biophysical chemistry POPC Diphenylhexatriene/chemistry Membranes lcsh:R technology industry and agriculture Green Chemistry Technology VIBRIO-FISCHERI VIABILITY 0104 chemical sciences Green chemistry chemistry Liposomes Ionic liquid Biophysics CHAIN lcsh:Q Biological fluorescence Ionic Liquids/chemistry Fluorescence anisotropy |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) Rantamäki, A H, Chen, W, Hyväri, P, Helminen, J, Partl, G, King, A W T & Wiedmer, S K 2019, ' Interactions of Ionic Liquids and Spirocyclic Compounds with Liposome Model Membranes. A Steady-State Fluorescence Anisotropy Study ', Scientific Reports, vol. 9, no. 1, 18349 . https://doi.org/10.1038/s41598-019-53893-w |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-53893-w |
| Popis: | Understanding the toxicity of ionic liquids (ILs) is crucial in the search of greener chemicals. By comparing in vivo toxicity and in vitro interactions determined between compounds and biomimetic lipid membranes, more detailed toxicity vs. structure relation can be obtained. However, determining the interactions between non-surface-active compounds and liposomes has been a challenging task. Organisational changes induced by ILs and IL-like spirocyclic compounds within 1,6-diphenyl-1,3,5-hexatriene-doped biomimetic liposomes was studied by steady-state fluorescence anisotropy technique. The extent of organisational changes detected within the liposome bilayers were compared to the toxicity of the compounds determined using Vibrio Fischeri bacteria. Four liposome compositions made of pure 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocoline (POPC) and mixtures of POPC, 1-palmitoyl-2-oleyl-sn-glycero-3-phosphoserine (POPS), and cholesterol (Chol) were tested as biomimetic models. Changes observed within the POPC/POPS/Chol 55:20:25 bilayers correlated the best with the toxicity results: ten out of twelve compounds followed the trend of increasing bilayer disorder – increasing toxicity. The study suggests that the toxicity of non-surface-active compounds is dependent on their ability to diffuse into the bilayers. The extent of bilayer’s organisational changes correlates rather well with the toxicity of the compounds. Highly sensitive technique, such as fluorescence anisotropy measurements, is needed for detecting subtle changes within the bilayer structures. |
| Databáze: | OpenAIRE |
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