| Autor: |
Woo-Gyun Choi, Jaeseok Han, Ji-Hyeon Kim, Kim, Mi-Jeong, Park, Jae-Woo, Benbo Song, Cha, Hee-Jeong, Hye-Seon Choi, Hun-Taeg Chung, Lee, In-Kyu, Park, Tae-Sik, Hatzoglou, Maria, Hueng-Sik Choi, Yoo, Hyun, Kaufman, Randal, Back, Sung |
| Rok vydání: |
2017 |
| DOI: |
10.6084/m9.figshare.c.3841105_d3.v1 |
| Popis: |
eIF2α phosphorylation in hepatocytes is dispensable for survival of adult mice. (a) Diagram depicting the four genotypes of mice used in these experiments. S/A and A/A represent heterozygous and homozygous eIF2α Ser51Ala (*) mutation(s) in exon 2 of one eIF2α allele and both eIF2α alleles, respectively. fTg/0 represents the floxed wild type (WT) eIF2α transgene driven by the CMV enhancer and chicken β-actin promoter (Enh-Pro). The loxP sequences (black arrowheads) allow excision of the WT eIF2α floxed transgene (fTg) and expression of EGFP, an indicator of recombination. CRE Hep /0 represents the Cre recombinase transgene driven by the promoter (Alfp) of Alb1 (encoding albumin) and the enhancer of Afp (encoding alpha-fetoprotein). (b) Efficiency of deletion of the fTg in liver tissues. Results from quantitative RT-PCR analyses of transgenic and total eIF2α mRNAs are shown. Data are means ± SEM (n = 4 ~ 5 mice per group); ### p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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