Effect of Drug Carrier Melting Points on Drug Release of Dexamethasone-Loaded Microspheres
| Autor: | Ji Yeon Heo, Ji Hoon Park, Jae-Ho Kim, Bong Lee, Seung Hun Park, Doo Yeon Kwon, Moon Suk Kim, Joon Yeong Park |
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| Rok vydání: | 2017 |
| Předmět: |
Biomedical Engineering
Medicine (miscellaneous) 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences In vitro 0104 chemical sciences PLGA chemistry.chemical_compound chemistry In vivo Melting point Copolymer Original Article Particle size Fluorescein 0210 nano-technology Drug carrier Nuclear chemistry Biomedical engineering |
| Zdroj: | Tissue engineering and regenerative medicine. 14(6) |
| ISSN: | 2212-5469 |
| Popis: | Here, we examined the effect of melting point of drug carriers on drug release of dexamethasone (Dex)-loaded microspheres. We prepared poly(L-lactide-ran-e-caprolactone) (PLC) copolymers with varying compositions of poly(e-caprolactone) (PCL) and poly(L-lactide) (PLLA). As the PLLA content increased, the melting points of PLC copolymers decreased from 61 to 43 °C. PLC copolymers in vials solubilized at 40–50 °C according to the incorporation of PLLA into the PCL segment. Dexamethasone (Dex)-loaded PLC (MCxLy) microspheres were prepared by the oil-in-water (O/W) solvent evaporation/extraction method. The preparation yields were above 70%, and the mean particle size ranged from 30 to 90 μm. The MCxLy microspheres also showed controllable melting points in the range of 40–60 °C. Dex-loaded MCxLy microspheres showed similar in vitro and in vivo sustained release patterns after the initial burst of Dex. The in vitro and in vivo order of the Dex release was MC80L20 > MC90L10 > MC95L5, which agreed well with the melting point order of the drug carrier. Using in vivo fluorescence imaging of fluorescein (FI)-loaded microspheres implanted in animals, we confirmed the sustained release of FI over an extended period. In vivo inflammation associated with the PLC microsphere implants was less pronounced than that associated with Poly(lactide-co-glycolide) (PLGA). In conclusion, we successfully demonstrated that it is possible to control Dex release using Dex-loaded MCxLy microspheres with different melting points. |
| Databáze: | OpenAIRE |
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