Enhancement of adhesive property of epithelial cell line Mm2T by culture in the presence of methylated vitamin B12
| Autor: | Masao Iwamori, Minoru Nakahara, Yukie Kitano, Kiyonori Harii, Toshiko Takaoka |
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| Rok vydání: | 1995 |
| Předmět: |
Physiology
Glycoconjugate Cell Communication Methylation Epithelium Cell Line medicine Cell Adhesion Animals Cell adhesion Molecular Biology chemistry.chemical_classification biology Chemistry Lectin Cell Biology General Medicine Adhesion Trypsin Molecular biology Phenotype Microscopy Electron Vitamin B 12 medicine.anatomical_structure Biochemistry biology.protein Microscopy Electron Scanning Immunohistochemistry medicine.drug |
| Zdroj: | Cell structure and function. 20(3) |
| ISSN: | 0386-7196 |
| Popis: | A thymic epithelial cell line Mm2T was cultured in a medium containing a high concentration (100 micrograms/ml) of methylated vitamin B12 (CH3-B12). After 19 days, cells were found to have a flat phenotype, to have lost the floating cells which were observed in the control cells at the confluent stage, and to have acquired a resistance to trypsin. However, treatment of the CH3-B12-treated cells with EDTA resulted in a dissociation of cell-to-cell contact and reaggregation was achieved by addition of Ca2+, indicating the involvement of Ca2+ ion in cell-to-cell contact. Electron microscopic analyses revealed that the CH3-B12-treated cells were nearly square in their vertical section, which was in contrast to the dome-shaped feature of the control cells, and their cell-to-cell contact area was significantly widespread, as compared to those of the control, indicating that Mm2T cells acquires an adhesive property by treatment with CH3-B12. Biochemical analyses of both cells indicated that the concentration of glucosylceramide in the CH3-B12-treated cells was higher than that of the control. Free glucose characteristically inhibited the reattachment of cells dissociated with EDTA, suggesting the involvement of glucose in the cell-to-cell adhesion of CH3-B12-treated cells. In addition, WGA-binding glycoconjugates were intensely observed in the boundary region of CH3-B12-treated cells by immunohistochemical staining, but not in that of the control cells. It is suggested that CH3-B12 may affect the morphological alteration of Mm2T by enhancing cell adhesion through elevated expression of the C-type lectin. |
| Databáze: | OpenAIRE |
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