Signal hotspot mutations in SARS-CoV-2 genomes evolve as the virus spreads and actively replicates in different parts of the world
Autor: | Walter Doerfler, Stefanie Weber, Christina M. Ramirez |
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Rok vydání: | 2020 |
Předmět: |
Cancer Research
viruses Questions about immunogenesis and vaccine development Mutant Sequence Homology Selection of viral hotspot mutations Global Health Virus Replication Genome Conserved sequence Russia Germany Peptide sequence Conserved Sequence Genetics Impact on replication-relevant viral proteins 0303 health sciences food and beverages Penetrance Biological Evolution Europe Infectious Diseases RNA Viral Sequence comparisons between 570 viral genomes to Wuhan isolate Coronavirus Infections congenital hereditary and neonatal diseases and abnormalities China Pneumonia Viral information science India Sequence alignment Genome Viral Biology Article Virus 03 medical and health sciences Betacoronavirus Virology Humans Amino Acid Sequence Pandemics 030304 developmental biology Base Sequence 030306 microbiology SARS-CoV-2 Consequences for secondary and tertiary structures of viral RNA Severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) COVID-19 biochemical phenomena metabolism and nutrition United States Viral replication Amino Acid Substitution Mutation Sequence Alignment |
Zdroj: | Virus Research |
ISSN: | 1872-7492 |
Popis: | Highlights • Study of SARS-CoV-2 genomes from world-wide isolates. • Sequence comparisons of 570 isolates to original Wuhan 2019 SARS-CoV-2 clade. • Identification of several hotspot mutants after world-wide spreading of virus. • Several hotspot mutations affect sequences of replication-relevant viral proteins. • How do hotspot mutations relate to viral pathogenicity? Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) was first identified in Wuhan, China late in 2019. Nine months later (Sept. 23, 2020), the virus has infected > 31.6 million people around the world and caused > 971.000 (3.07 %) fatalities in 220 countries and territories. Research on the genetics of the SARS-CoV-2 genome, its mutants and their penetrance can aid future defense strategies. By analyzing sequence data deposited between December 2019 and end of May 2020, we have compared nucleotide sequences of 570 SARS-CoV-2 genomes from China, Europe, the US, and India to the sequence of the Wuhan isolate. During worldwide spreading among human populations, at least 10 distinct hotspot mutations had been selected and found in up to > 80 % of viral genomes. Many of these mutations led to amino acid exchanges in replication-relevant viral proteins. Mutations in the SARS-CoV-2 genome would also impinge upon the secondary structure of the viral RNA molecule and its repertoire of interactions with essential cellular and viral proteins. The increasing frequency of SARS-CoV-2 mutation hotspots might select for dangerous viral pathogens. Alternatively, in a 29.900 nucleotide-genome, there might be a limit to the number of mutable and selectable sites which, when exhausted, could prove disadvantageous to viral survival. The speed, at which novel SARS-CoV-2 mutants are selected and dispersed around the world, could pose problems for the development of vaccines and therapeutics. |
Databáze: | OpenAIRE |
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