Recombinant Human Adiponectin as a Potential Protein for Treating Diabetic Tendinopathy Promotes Tenocyte Progenitor Cells Proliferation and Tenogenic Differentiation In Vitro
| Autor: | Hussin A. Rothan, Abdulrazzaq Mahmod Suhaeb, Tunku Kamarul |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
tenogenic differentiation medicine.medical_treatment Cellular differentiation Blotting Western In Vitro Techniques Biology Real-Time Polymerase Chain Reaction Tendons Tenocyte progenitor cells Internal medicine medicine Humans Tissue engineering Cloning Molecular Progenitor cell Cell Proliferation Adiponectin Gene Expression Profiling Stem Cells Insulin Scleraxis diabetic tendinopathy Cell Differentiation General Medicine Recombinant Proteins Tenomodulin Endocrinology Diabetes Mellitus Type 2 Adipogenesis Tendinopathy Electrophoresis Polyacrylamide Gel Stem cell Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| DOI: | 10.7150/ijms.6774 |
| Popis: | Adiponectin is an adipocyte-secreting hormone that increases cell sensitivity to insulin. It has been previously demonstrated that this hormone protects against Type II Diabetes and, is found to concurrently promote cell proliferation and differentiation. It is postulated that diabetic patients who suffer from tendinopathy may benefit from using adiponectin, which not only improves the metabolism of diabetic ridden tenocytes but also promotes progenitor cell proliferation and differentiation in tendons. These changes may result in tendon regeneration, which, in diabetic tendinopathy, is difficult to treat. Considering that such findings have yet to be demonstrated, a study was thus conducted using diabetic ridden human tenocyte progenitor cells (TPC) exposed to recombinant adiponectin in vitro. TPC were isolated from tendons of diabetic patients and exposed to 10μg/ml adiponectin. Cell proliferation rate was investigated at various time points whilst qPCR were used to determine the tenogenic differentiation potential. The results showed that adiponectin significantly reduced blood glucose in animal models. The proliferation rate of adiponectin-treated TPCs was significantly higher at 6, 8 and 10 days as compared to untreated cells (p |
| Databáze: | OpenAIRE |
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