Influence of water temperature, salinity, and pH on survival and growth of toxigenic Vibrio cholerae serovar 01 associated with live copepods in laboratory microcosms
| Autor: | M I Huq, P A West, Rita R. Colwell, E B Small, Anwarul Huq |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Biology
medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Agar plate medicine Animals Vibrio cholerae Ecology Osmolar Concentration fungi Temperature Hydrogen-Ion Concentration Plankton biology.organism_classification medicine.disease Crustacean Cholera Salinity Kinetics Microscopy Electron Scanning Water Microbiology Microcosm Bacteria Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 48:420-424 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.48.2.420-424.1984 |
| Popis: | The influence of water temperature, salinity, and pH on the multiplication of toxigenic Vibrio cholerae serovar O1 cells and their attachment to live planktonic crustaceans, i.e., copepods, was investigated by using laboratory microcosms. By increasing water temperatures up to 30 degrees C, a pronounced effect on the multiplication of V. cholerae was demonstrated, as was attachment of the cells to live copepods. These were measured by culturable counts on agar plates and direct observation by scanning electron microscopy, respectively. Of the three salinities examined (5, 10, and 15%), maximum growth of V. cholerae and attachment to copepods occurred at 15%. An alkaline pH (8.5) was optimal both for attachment and multiplication of V. cholerae, as compared with pH 6.5 and 7.5. It is concluded that conditions affecting attachment of V. cholerae serovar O1 to live copepods observed under laboratory conditions may also occur in the natural estuarine environment and, thereby, are significant in the epidemiology of cholera. |
| Databáze: | OpenAIRE |
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