Coco is a dual-activity modulator of TGF-β signaling
Autor: | Ali H. Brivanlou, Aryeh Warmflash, Alessia Deglincerti, Benoit Sorre, Tomomi Haremaki |
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Rok vydání: | 2015 |
Předmět: |
Immunoprecipitation
Xenopus Receptor Transforming Growth Factor-beta Type I Regulator Fluorescent Antibody Technique Protein Serine-Threonine Kinases Xenopus Proteins Biology Real-Time Polymerase Chain Reaction Bioinformatics Transforming Growth Factor beta1 RNA interference Animals Coco RNA Small Interfering Luciferases Enhancer Receptor Molecular Biology DNA Primers Reverse Transcriptase Polymerase Chain Reaction biology.organism_classification Cell biology RNA Interference Signal transduction Receptors Transforming Growth Factor beta Signal Transduction Research Article Developmental Biology |
Zdroj: | Development. |
ISSN: | 1477-9129 0950-1991 |
Popis: | The TGFβ signaling pathway is a critical regulator of developmental processes and disease. The activity of TGFβ ligands is modulated by various families of soluble inhibitors that interfere with the interactions between ligands and receptors. In an unbiased, genome-wide RNAi screen to identify genes involved in ligand-dependent signaling, we unexpectedly identified the BMP/Activin/Nodal inhibitor Coco as an enhancer of TGFβ1 signaling. Coco synergizes with TGFβ1 in both cell culture and Xenopus explants. Molecularly, Coco binds to TGFβ1 and enhances TGFβ1 binding to its receptor Alk5. Thus, Coco acts both as an inhibitor and an enhancer of signaling depending on the ligand it binds. This finding raises the need for a global reconsideration of the molecular mechanisms regulating TGFβ signaling. |
Databáze: | OpenAIRE |
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