The revelation of expressing region in the processed ceruloplasmin gene in human genome by biocomputational and biochemical methods
| Autor: | N.A. Platonova, N.V. Tsymbalenko, L. V. Puchkova, S. A. Klotchenko, Andrey V. Vasin |
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| Rok vydání: | 2005 |
| Předmět: |
Models
Molecular Signal peptide Pseudogene Molecular Sequence Data Biophysics Models Biological Biochemistry Cell Line Tumor Humans Amino Acid Sequence Gene Peptide sequence chemistry.chemical_classification biology Computers Genome Human Chemistry Organic Chemistry Ceruloplasmin Computational Biology Molecular biology Protein Structure Tertiary Amino acid Open reading frame biology.protein Human genome Sequence Alignment Pseudogenes |
| Zdroj: | Biophysical Chemistry. 115:247-250 |
| ISSN: | 0301-4622 |
| DOI: | 10.1016/j.bpc.2004.12.014 |
| Popis: | Annotation Translation in all open reading frames (ORF) of human ceruloplasmin (Cp) pseudogene revealed the only translating sequence of 984 nucleotides. The amino acid sequence contains a signal peptide for mitochondrial protein import at N-terminus. The predicted protein without taking the signal peptide into consideration has 92% identity to the corresponding Cp fragment. It contains 20 amino acid substitutions, 8 of them are significant. There is His-X-His motif in the center of a molecule that is typical for copper containing oxidases. Potential copper-binding site appears as a result of the substitution P923H along human Cp sequence. Cp pseudogene transcription product was found in the cultured human cell lines HepG2 and HuTu 80 using RT-PCR strategy. Cp polypeptides with molecular weight of nearly 30 kDa were found in mitochondria of HuTu 80 cells. The possible biological role of mitochondrial Cp is under discussion. |
| Databáze: | OpenAIRE |
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