Elevated seminal protein carbonyl concentration is correlated with asthenozoospermia and affects adversely the laboratory intracytoplasmic sperm injection (ICSI) outcomes
| Autor: | Osamah Batiha, Mohammad A Al Smadi, Houda Amor, Emad Al Sharu, Mohammad Y Jahmani, Mohamad Eid Hammadeh, Ahmed Mahdy, Mohammad M. Al-Talib |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Urology medicine.medical_treatment Semen Oxidative phosphorylation Asthenozoospermia Intracytoplasmic sperm injection Protein Carbonylation Andrology Endocrinology medicine Humans Sperm Injections Intracytoplasmic reproductive and urinary physiology Fertilisation Sperm motility chemistry.chemical_classification Reactive oxygen species urogenital system Chemistry General Medicine medicine.disease Spermatozoa Sperm Motility Laboratories Embryo quality |
| Zdroj: | Andrologia. 53 |
| ISSN: | 1439-0272 0303-4569 |
| DOI: | 10.1111/and.14232 |
| Popis: | Elevated concentrations of reactive oxygen species (ROS) in the semen can lead to oxidative protein damage as they react with the amino acids' side chains in the protein, leading to the generation of carbonyl groups. This study aimed to investigate the effect of protein carbonyl (PC) concentration on sperm motility and the laboratory intracytoplasmic sperm injection (ICSI) outcomes. A total of 150 couples from the ICSI cycle were enrolled in this study and were divided into three groups (G) according to the PC concentration as following, G1 included samples with PC concentrations0.65 nmol/mg, G2 included samples with 0.65≤PC≤2.23 nmol/mg and G3 included samples with PC2.23 (nmol/mg). PC concentrations were measured in all semen samples, and the laboratory ICSI outcomes were evaluated for all injected oocytes. The Kruskal-Wallis p-values for the differences in the medians of sperm motility, fertilisation rate, embryo cleavage score and embryo quality score were0.05. Furthermore, Dunn's post hoc test showed a significant difference between all groups, p-values0.05, except for the medians of embryo quality score between G2 and G3. In conclusion, our results showed that sperm motility and laboratory ICSI outcomes are affected negatively by higher concentrations of PC in the semen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|