Self-assembly formation of multiple DNA-tethered lipid bilayers
| Autor: | Peter Jönsson, Magnus Brändén, Seyed R. Tabaei, Fredrik Höök |
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| Rok vydání: | 2009 |
| Předmět: |
Lipid Bilayers
Analytical chemistry Fluorescence recovery after photobleaching DNA Quartz crystal microbalance Models Theoretical Model lipid bilayer chemistry.chemical_compound Membrane chemistry Structural Biology Phosphatidylcholine Fluorescence microscope Biophysics lipids (amino acids peptides and proteins) Self-assembly Dimyristoylphosphatidylcholine Lipid bilayer |
| Zdroj: | Journal of Structural Biology. 168:200-206 |
| ISSN: | 1047-8477 |
| DOI: | 10.1016/j.jsb.2009.07.008 |
| Popis: | Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid-DNA architecture. |
| Databáze: | OpenAIRE |
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