A Single LC-MS/MS Analysis to Quantify CoA Biosynthetic Intermediates and Short-Chain Acyl CoAs
| Autor: | David Meriwether, Nataly J. Arias, Anthony E. Jones, Aracely Acevedo, Srinivasa T. Reddy, Ajit S. Divakaruni |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism Coenzyme A Clinical Sciences Mitochondrion Mass spectrometry Microbiology 01 natural sciences Biochemistry Cofactor Article Analytical Chemistry etomoxir 03 medical and health sciences Acyl-CoA chemistry.chemical_compound CoA biosynthesis Solid phase extraction LC-MS/MS Molecular Biology biology 010401 analytical chemistry short-chain acyl CoAs QR1-502 0104 chemical sciences mitochondria 030104 developmental biology chemistry biology.protein Biochemistry and Cell Biology Intracellular Etomoxir |
| Zdroj: | Metabolites Metabolites, vol 11, iss 8 Metabolites, Vol 11, Iss 468, p 468 (2021) Volume 11 Issue 8 |
| ISSN: | 2218-1989 |
| Popis: | Coenzyme A (CoA) is an essential cofactor for dozens of reactions in intermediary metabolism. Dysregulation of CoA synthesis or acyl CoA metabolism can result in metabolic or neurodegenerative disease. Although several methods use liquid chromatography coupled with mass spectrometry/mass spectrometry (LC-MS/MS) to quantify acyl CoA levels in biological samples, few allow for simultaneous measurement of intermediates in the CoA biosynthetic pathway. Here we describe a simple sample preparation and LC-MS/MS method that can measure both short-chain acyl CoAs and biosynthetic precursors of CoA. The method does not require use of a solid phase extraction column during sample preparation and exhibits high sensitivity, precision, and accuracy. It reproduces expected changes from known effectors of cellular CoA homeostasis and helps clarify the mechanism by which excess concentrations of etomoxir reduce intracellular CoA levels. |
| Databáze: | OpenAIRE |
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