The anti-inflammatory and anti-oxidative actions of eugenol improve lipopolysaccharide-induced lung injury
| Autor: | Walter A. Zin, Nathalia V. Casquilho, José Henrique Leal-Cardoso, Rodrigo S. Fortunato, Leonardo H. Travassos, Mariana Nascimento Machado, Débora S. Faffe, D. R. Riva, Clarissa Bichara Magalhães |
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| Rok vydání: | 2018 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lipopolysaccharides Male Antioxidant Physiology medicine.medical_treatment Anti-Inflammatory Agents Pharmacology Lung injury Protein oxidation medicine.disease_cause Antioxidants Statistics Nonparametric Proinflammatory cytokine Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Eugenol medicine Pulmonary Medicine Animals chemistry.chemical_classification Inflammation Mice Inbred BALB C biology Dose-Response Relationship Drug General Neuroscience Glutathione peroxidase NADPH Oxidases Lung Injury Disease Models Animal Oxidative Stress 030228 respiratory system chemistry biology.protein Cytokines 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Respiratory physiologyneurobiology. 259 |
| ISSN: | 1878-1519 |
| Popis: | Acute lung injury (ALI) remains a major cause of mortality. In lipopolysaccharide (LPS)-stimulated macrophages, eugenol reduces cyclooxygenase-2 expression, NF-κB activation, and inflammatory mediators. We examined the anti-inflammatory and anti-oxidative action of eugenol in an in vivo model of LPS-induced lung injury. Lung mechanics and histology were analyzed in mice 24 h after LPS exposure, with and without eugenol treatment at different doses. Additional animals, submited to the same protocol, were treated with eugenol at 150 mg/kg to determine its effect on inflammatory cytokines (ELISA) and oxidative markers. LPS-induced lung functional and histological changes were significantly improved by eugenol, in a dose-dependent way. Furthermore, eugenol (150 mg/kg) was able to inhibit the release of inflammatory cytokines (TNF-α, IL-1β and IL-6), NADPH oxidase activity, as well as antioxidant enzymes activity (superoxide dismutase, catalase and glutathione peroxidase). Finally, eugenol reduced LPS-induced protein oxidation. In conclusion, eugenol improved in vivo LPS-induced ALI through both anti-inflammatory and anti-oxidative effects, avoiding damage to lung structure. |
| Databáze: | OpenAIRE |
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