Golgi fragmentation in pmn mice is due to a defective ARF1/TBCE cross-talk that coordinates COPI vesicle formation and tubulin polymerization
| Autor: | Dorothée Buttigieg, Gilbert Baillat, Catherine Rabouille, Georg Haase, Michael K. E. Schäfer, Sarah Bellouze |
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| Přispěvatelé: | Hubrecht Institute for Developmental Biology and Stem Cell Research |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Vesicle fusion
Golgi Apparatus Coat Protein Complex I Polymerization Muscular Atrophy Spinal Mice symbols.namesake Tubulin Microtubule Genetics medicine Animals Humans Fragmentation (cell biology) Molecular Biology Genetics (clinical) Motor Neurons biology Vesicle Amyotrophic Lateral Sclerosis General Medicine COPI Motor neuron Golgi apparatus Cell biology Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure biology.protein symbols ADP-Ribosylation Factor 1 COP-Coated Vesicles Molecular Chaperones Signal Transduction |
| Zdroj: | Human Molecular Genetics, 23(22), 5961-75. Oxford University Press |
| ISSN: | 0964-6906 |
| Popis: | Golgi fragmentation is an early hallmark of many neurodegenerative diseases but its pathophysiological relevance and molecular mechanisms are unclear. We here demonstrate severe and progressive Golgi fragmentation in motor neurons of progressive motor neuronopathy (pmn) mice due to loss of the Golgi-localized tubulin-binding cofactor E (TBCE). Loss of TBCE in mutant pmn and TBCE-depleted motor neuron cultures causes defects in Golgi-derived microtubules, as expected, but surprisingly also reduced levels of COPI subunits, decreased recruitment of tethering factors p115/GM130 and impaired Golgi SNARE-mediated vesicle fusion. Conversely, ARF1, which stimulates COPI vesicle formation, enhances the recruitment of TBCE to the Golgi, increases polymerization of Golgi-derived microtubules and rescues TBCE-linked Golgi fragmentation. These data indicate an ARF1/TBCE-mediated cross-talk that coordinates COPI formation and tubulin polymerization at the Golgi. We conclude that interruption of this cross-talk causes Golgi fragmentation in pmn mice and hypothesize that similar mechanisms operate in human amyotrophic lateral sclerosis and spinal muscular atrophy. |
| Databáze: | OpenAIRE |
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